Team:BIOSINT Mexico/Results

From 2013.igem.org

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*The sample of the lane 5 is the Plasmid GFP
*The sample of the lane 5 is the Plasmid GFP
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Revision as of 02:52, 28 September 2013

SmartPro

Smartpro

Igem LogotipoDefinitivo



Results



Piezasgens.jpg

Plasmid extraction from synthetized parts

pUC57: 2710 bp

  • In the lane 1 is the ladder of 5000 bp
  • The sample of the lane 3 is the promoter 1, inserted sequence: 103 bp
  • The sample of the lane 5 is the promoter 2, inserted sequence: 103 bp
  • The sample of the lane 7 is the RBS, inserted sequence: 76 bp













Piezas PsB1C3.jpg



Parts clonning in pSB1C3: 2070 bp


  • In the lane 1 is the ladder of 3000 bp
  • The sample of the lane 3 is the promoter 2
  • The sample of the lane 4 is the promoter 1
  • The sample of the lane 7 is the RBS












Plasmid GFP.jpg



Ligation Promoters with GFP


  • In the lane 1 is the ladder of 5000 bp
  • The sample of the lane 3 is the Plasmid GFP
  • The sample of the lane 5 is the Plasmid GFP











P1 expression.jpg


GFP expression for L.Plantarum promoter cloned in E.coli