http://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&feed=atom&action=historyTeam:BIOSINT Mexico/Safety: Kill switch - Revision history2024-03-28T14:00:29ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=241848&oldid=prevJuan noe at 03:49, 28 September 20132013-09-28T03:49:45Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:49, 28 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 16:</td>
<td colspan="2" class="diff-lineno">Line 16:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>So our construct could be DOUBLE SECURITY because it is strain specific and function specific.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>So our construct could be DOUBLE SECURITY because it is strain specific and function specific.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Plantaricins===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Plantaricins===</div></td></tr>
</table>Juan noehttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=241746&oldid=prevJuan noe: /* Specific kill switch */2013-09-28T03:48:02Z<p><span class="autocomment">Specific kill switch</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:48, 28 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 8:</td>
<td colspan="2" class="diff-lineno">Line 8:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Specific kill switch===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Specific kill switch===</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[File:Veneno.png|180px|left]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:As an extra safety measure we adapt an specific kill switch in order than our probiotic was only capable to make the specific function for wich was design. This mechanism can be regulated through a sensor, so it will be activated only when it is desired. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:As an extra safety measure we adapt an specific kill switch in order than our probiotic was only capable to make the specific function for wich was design. This mechanism can be regulated through a sensor, so it will be activated only when it is desired. </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Plasmid recombination occurs naturally on the microbiota, so apply a kill switch could be dangerous for the others bacterial in the intestine. For that reason we development the concept of specificity, using a method that only could act in our lactobacillus strain. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Plasmid recombination occurs naturally on the microbiota, so apply a kill switch could be dangerous for the others bacterial in the intestine. For that reason we development the concept of specificity, using a method that only could act in our lactobacillus strain. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>So our construct could be DOUBLE SECURITY because it is strain specific and function specific. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>So our construct could be DOUBLE SECURITY because it is strain specific and function specific.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Plantaricins===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Plantaricins===</div></td></tr>
</table>Juan noehttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=240209&oldid=prevGabyqz: /* Plantaricins */2013-09-28T03:27:22Z<p><span class="autocomment">Plantaricins</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:27, 28 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 31:</td>
<td colspan="2" class="diff-lineno">Line 31:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[File:ks.JPG|center|200px]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Safety forms===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Safety forms===</div></td></tr>
</table>Gabyqzhttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=231040&oldid=prevRicardo Cuenca at 00:50, 28 September 20132013-09-28T00:50:16Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:50, 28 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 13:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Plasmid recombination occurs naturally on the microbiota, so apply a kill switch could be dangerous for the others bacterial in the intestine. For that reason we development the concept of specificity, using a method that only could act in our lactobacillus strain. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Plasmid recombination occurs naturally on the microbiota, so apply a kill switch could be dangerous for the others bacterial in the intestine. For that reason we development the concept of specificity, using a method that only could act in our lactobacillus strain. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>So our construct could be DOUBLE <del class="diffchange diffchange-inline">SECURETY </del>because is strain specific and function specific. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>So our construct could be DOUBLE <ins class="diffchange diffchange-inline">SECURITY </ins>because <ins class="diffchange diffchange-inline">it </ins>is strain specific and function specific. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Ricardo Cuencahttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=226644&oldid=prevJuan noe at 23:15, 27 September 20132013-09-27T23:15:08Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 23:15, 27 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 31:</td>
<td colspan="2" class="diff-lineno">Line 31:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">===Safety forms===</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Juan noehttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=219743&oldid=prevJuan noe at 20:20, 27 September 20132013-09-27T20:20:51Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:20, 27 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 6:</td>
<td colspan="2" class="diff-lineno">Line 6:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">=== Specific kill switch===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">:As an extra safety measure we adapt an specific kill switch in order than our probiotic was only capable to make the specific function for wich was design. This mechanism can be regulated through a sensor, so it will be activated only when it is desired. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">:Plasmid recombination occurs naturally on the microbiota, so apply a kill switch could be dangerous for the others bacterial in the intestine. For that reason we development the concept of specificity, using a method that only could act in our lactobacillus strain. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">So our construct could be DOUBLE SECURETY because is strain specific and function specific. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 23:</td>
<td colspan="2" class="diff-lineno">Line 31:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">:This mechanism can be regulated through a sensor, so it will be activated only when it is desired.</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
</table>Juan noehttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=217660&oldid=prevJuan noe at 19:27, 27 September 20132013-09-27T19:27:16Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:27, 27 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 9:</td>
<td colspan="2" class="diff-lineno">Line 9:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Plantaricins===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Plantaricins===</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">----</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Bacteriocins are ribosomally synthetized antimicrobial peptides produced by bacteria.4 Unlike antibiotics, bacteriocins have a narrow killing spectrum and affect only closely related strains.7 They can be classified in different groups depending on how they are produced, their components, or the bacteria they affect.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Bacteriocins are ribosomally synthetized antimicrobial peptides produced by bacteria.4 Unlike antibiotics, bacteriocins have a narrow killing spectrum and affect only closely related strains.7 They can be classified in different groups depending on how they are produced, their components, or the bacteria they affect.</div></td></tr>
</table>Juan noehttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=217641&oldid=prevJuan noe at 19:26, 27 September 20132013-09-27T19:26:58Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:26, 27 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 21:</td>
<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>:Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This mechanism can be regulated through a sensor, so it will be activated only when it is desired.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">:</ins>This mechanism can be regulated through a sensor, so it will be activated only when it is desired.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
</table>Juan noehttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=217624&oldid=prevJuan noe at 19:26, 27 September 20132013-09-27T19:26:22Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 19:26, 27 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 10:</td>
<td colspan="2" class="diff-lineno">Line 10:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Plantaricins===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Plantaricins===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Bacteriocins are ribosomally synthetized antimicrobial peptides produced by bacteria.4 Unlike antibiotics, bacteriocins have a narrow killing spectrum and affect only closely related strains.7 They can be classified in different groups depending on how they are produced, their components, or the bacteria they affect.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">:</ins>Bacteriocins are ribosomally synthetized antimicrobial peptides produced by bacteria.4 Unlike antibiotics, bacteriocins have a narrow killing spectrum and affect only closely related strains.7 They can be classified in different groups depending on how they are produced, their components, or the bacteria they affect.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Plantaricins are one kind of bacteriocins which belong to the IIB group. This means they are heat stable, postranslationally unmodified non-lantibiotics, and are formed by two peptides. As their name suggests, they are produced by Lactobacillus plantarum.2</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Recent studies have been developed on the identification of plantaricins, their activity, and interactions among each other.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">:</ins>Plantaricins are one kind of bacteriocins which belong to the IIB group. This means they are heat stable, postranslationally unmodified non-lantibiotics, and are formed by two peptides. As their name suggests, they are produced by Lactobacillus plantarum.2</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Plantaricin A (PlnA) is a peptide that has both bacteriocin and pheromone activity.5 When it is produced, Plantaricin A activates five operons which are identified as plnABCD, plnEFI, plnJKLR, plnMNOP, and plnGHSTUV. The first operon codifies for pln A itself and for several proteins involved in the transduction process. Both pln EFI and pln JKLR codify for the two peptide Plantaricins. PlnE interacts with PlnF to form PlnEF while PlnJ interacts with PlnK to form PlnJK. Research suggests that certain types of strains are more susceptible to PlnEF, and others to PlnJK.1 The other subunits of this operon codify for immunity-like proteins that protect the producer against its own plantaricins. The activity of the fourth operon is still unknown, and the fifth operon has two ORF’s, one which results in PlnGH which encodes the machinery responsible of secreting and processing the Plantaricin precursors.3</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>PlnEF and PlnJK have strain specific antagonist activity, which is optimum when both subunits are produced equally.1 As part of our project, we will try to create a killing mechanism which produces a plantaricin (either one or the other) without producing the immunity proteins so our Lactobacillus will be susceptible to these substances once they are outside the cell membrane. As they are strain specific, they will not affect the other members of the human microbiota, nor will they be a potential risk for human health.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">:</ins>Recent studies have been developed on the identification of plantaricins, their activity, and interactions among each other.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">:</ins>Plantaricin A (PlnA) is a peptide that has both bacteriocin and pheromone activity.5 When it is produced, Plantaricin A activates five operons which are identified as plnABCD, plnEFI, plnJKLR, plnMNOP, and plnGHSTUV. The first operon codifies for pln A itself and for several proteins involved in the transduction process. Both pln EFI and pln JKLR codify for the two peptide Plantaricins. PlnE interacts with PlnF to form PlnEF while PlnJ interacts with PlnK to form PlnJK. Research suggests that certain types of strains are more susceptible to PlnEF, and others to PlnJK.1 The other subunits of this operon codify for immunity-like proteins that protect the producer against its own plantaricins. The activity of the fourth operon is still unknown, and the fifth operon has two ORF’s, one which results in PlnGH which encodes the machinery responsible of secreting and processing the Plantaricin precursors.3</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">:</ins>PlnEF and PlnJK have strain specific antagonist activity, which is optimum when both subunits are produced equally.1 As part of our project, we will try to create a killing mechanism which produces a plantaricin (either one or the other) without producing the immunity proteins so our Lactobacillus will be susceptible to these substances once they are outside the cell membrane. As they are strain specific, they will not affect the other members of the human microbiota, nor will they be a potential risk for human health.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">:</ins>Generally speaking plantaricin will be produced inside the cell and exported outside the cell through ABC transporters.6 Once outside the cell, they will adopt an amphiphilic alpha helical structure which will allow them to interact with the cell membrane and permeabilize it.5 Up until now, the only known facts are that these plantaricins create pores in the membrane and dissipate both the transmembrane electrical potential (∆Ψ) and ∆pH gradient.6</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This mechanism can be regulated through a sensor, so it will be activated only when it is desired.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This mechanism can be regulated through a sensor, so it will be activated only when it is desired.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 25:</td>
<td colspan="2" class="diff-lineno">Line 30:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1Anderssen, Erlend L.; Diep, Dzung Bao; Nes, Ingolf F.; Eijsink, Vincent G. H. and Nissen-Meyer, Jon. (1998). Antagonistic Activity of Lactobacillus plantarum C11: Two New Two-Peptide Bacteriocins, Plantaricins EF and JK, and the Induction Factor Plantaricin A. [Online]. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC106311/ on April 10, 2013.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1Anderssen, Erlend L.; Diep, Dzung Bao; Nes, Ingolf F.; Eijsink, Vincent G. H. and Nissen-Meyer, Jon. (1998). Antagonistic Activity of Lactobacillus plantarum C11: Two New Two-Peptide Bacteriocins, Plantaricins EF and JK, and the Induction Factor Plantaricin A. [Online]. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC106311/ on April 10, 2013.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2Cintas, L.M.; Casaus, M.P.; Herranz, C.; Nes, I.F. and Hernández, P.E. (2013). Review: Bacteriocins of Lactic Acid Bacteria. [Online]. Retrieved from http://fst.sagepub.com/content/7/4/281.abstract on April 10, 2013.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2Cintas, L.M.; Casaus, M.P.; Herranz, C.; Nes, I.F. and Hernández, P.E. (2013). Review: Bacteriocins of Lactic Acid Bacteria. [Online]. Retrieved from http://fst.sagepub.com/content/7/4/281.abstract on April 10, 2013.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3Diep, D. B.; Havarstein, L. S. and Nes, I.F. (1996). Characterization of the locus responsible for the bacteriocin production in Lactobacillus plantarum C11. [Online]. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC178213/ on April 10, 2013.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3Diep, D. B.; Havarstein, L. S. and Nes, I.F. (1996). Characterization of the locus responsible for the bacteriocin production in Lactobacillus plantarum C11. [Online]. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC178213/ on April 10, 2013.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4Dobson, A., Cotter, P.D., Ross, R.P., Hill, C. (2012) Bacteriocin Production: a Probiotic Trait? [On-line] Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255625/ on April, 10, 2013.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4Dobson, A., Cotter, P.D., Ross, R.P., Hill, C. (2012) Bacteriocin Production: a Probiotic Trait? [On-line] Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3255625/ on April, 10, 2013.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5Hildeng Hauge, H., Mantzilas, D., Eijsink, V.G.H., Nissen-Meyer, J. (1999) Membrane-Mimicking Entities Induce Structuring of the Two-Peptide Bacteriocins Plantaricin E/F and Plantaricin J/K. [On-line] Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93438/ on April, 10, 2013.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>5Hildeng Hauge, H., Mantzilas, D., Eijsink, V.G.H., Nissen-Meyer, J. (1999) Membrane-Mimicking Entities Induce Structuring of the Two-Peptide Bacteriocins Plantaricin E/F and Plantaricin J/K. [On-line] Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93438/ on April, 10, 2013.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>6Moll, Gert N.; van den Akker, Emile; Hauge, Havard H.; Nissen-Meyer, Jon; Nes, Ingolf F.; Konings, Wil N. and Driessen, Arnold J. M. (1999). Complementary and Overlapping Selectivity of the Two-Peptide Bacteriocins Plantaricin EF and JK. [Online]. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93971/ on April 10, 2013.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>6Moll, Gert N.; van den Akker, Emile; Hauge, Havard H.; Nissen-Meyer, Jon; Nes, Ingolf F.; Konings, Wil N. and Driessen, Arnold J. M. (1999). Complementary and Overlapping Selectivity of the Two-Peptide Bacteriocins Plantaricin EF and JK. [Online]. Retrieved from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93971/ on April 10, 2013.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>7Riley, M.A., Wertz,J.E. (2002) BACTERIOCINS: Evolution, Ecology, and Application. [On-line] Retrieved from http://www.bio.umass.edu/biology/riley/sites/www.bio.umass.edu.biology.riley/files/2002c%20Bacteriocins-%20Evolution,%20Ecology,%20and%20Application_002.pdf on April, 10, 2013.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>7Riley, M.A., Wertz,J.E. (2002) BACTERIOCINS: Evolution, Ecology, and Application. [On-line] Retrieved from http://www.bio.umass.edu/biology/riley/sites/www.bio.umass.edu.biology.riley/files/2002c%20Bacteriocins-%20Evolution,%20Ecology,%20and%20Application_002.pdf on April, 10, 2013.</div></td></tr>
</table>Juan noehttp://2013.igem.org/wiki/index.php?title=Team:BIOSINT_Mexico/Safety:_Kill_switch&diff=208854&oldid=prevMague at 14:50, 27 September 20132013-09-27T14:50:34Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 14:50, 27 September 2013</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 8:</td>
<td colspan="2" class="diff-lineno">Line 8:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Plantaricins</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===</ins>Plantaricins<ins class="diffchange diffchange-inline">===</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Bacteriocins are ribosomally synthetized antimicrobial peptides produced by bacteria.4 Unlike antibiotics, bacteriocins have a narrow killing spectrum and affect only closely related strains.7 They can be classified in different groups depending on how they are produced, their components, or the bacteria they affect.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Bacteriocins are ribosomally synthetized antimicrobial peptides produced by bacteria.4 Unlike antibiotics, bacteriocins have a narrow killing spectrum and affect only closely related strains.7 They can be classified in different groups depending on how they are produced, their components, or the bacteria they affect.</div></td></tr>
</table>Mague