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From 2013.igem.org
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Revision as of 14:31, 22 September 2013
| A Brief Introduction of Integrated Sensor for Detection of Milk Product |
| As we know, antibiotics are widely used in modern industry to prevent the infection of cow breasts. However, the residual of antibiotics and other components, such as Cr (VI) which is widely used in the recycle of proteins from leather, will endanger the health of customers. Therefore, it is necessary to find solution to identify these harmful chemicals, but the method of timely detection of harmful chemicals in products still remains unsolved. To solve this problem, our sensor has been divided into three, respectively, of hexavalent chromium, tetracycline and β-lactam there is a response, for each sensor, we are all assembled in a downstream of the amplification block (amplifier) and the control block (controller), the output signal to be amplified, by adjusting the concentration of IPTG to achieve the adjustment of magnification. Amplifier by the T7 RNA polymerase gene and T7 promoter composition control block is through the downstream of the T7 promoter plus lacO and the addition of regular expression lacI, by adjusting the concentration of IPTG, lacO then adjust the boot situation achieved, the final response module for the green fluorescent protein. In order to achieve real-time detection, we also made a biochip which provided a reaction room for both engineering bacteria and samples. Now we are able to detect all these three harmful chemicals with a hand-held electronic equipment made by ourselves. |
