Team:BYU Provo/Notebook/Phage Purification/Fallexp/Period1/Explist

From 2013.igem.org

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This procedure purifies mutated T7 phage from the small phage team.
This procedure purifies mutated T7 phage from the small phage team.
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Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient|9.7 CsCl Gradient]]
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: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.76CsClGradient|9.7 CsCl Gradient]]
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We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.
We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.
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:-[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
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: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.910XPhageSuspensionBuffer| 9.9 10X Phage Suspension Buffer Creation]]
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- We ran another T7 phage purification procedure with new PSB.
- We ran another T7 phage purification procedure with new PSB.
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: -[[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]]
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: Detailed procedure: [[Team:BYU_Provo/Notebook/Phage_Purification/Fallexp/Period1/Exp/9.11CsClGradient| 9.11 CsCl Gradient]]

Revision as of 00:41, 28 September 2013


Phage Purification August - September Notebook: September 1 - September 14 Experiment Listing



Phage Purification
March-April
May-June
July-August
September-October

9.7 CsCl Gradient

This procedure purifies mutated T7 phage from the small phage team.

Detailed procedure: 9.7 CsCl Gradient


9.9 10X Phage Suspension Buffer Creation

We decided that our problem T7 banding in our gradient could be due to faulty phage suspension buffer. We created a new 10X solution of it to do all further gradients.

Detailed procedure: 9.9 10X Phage Suspension Buffer Creation


9/11/13

- We ran another T7 phage purification procedure with new PSB.

Detailed procedure: 9.11 CsCl Gradient