Team:BYU Provo/Notebook/Phage Purification/Summerexp

From 2013.igem.org


Phage Purification July - August Notebook



Phage Purification
March-April
May-June
July-August
September-October

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July 1 - July 15


We are working on getting Electron Microscope images of our purified phage.


Daily log

Experiment Listing


Manyphage.JPG
July 16 - July 31


In these two weeks, we ran a CsCl gradient for the T4 group. We also discussed the children's book and other team projects.


Daily log

Experiment Listing



PhageCircle.JPG


August 1 - August 16


These two weeks were spent focussing on isolating T7 phage. After successful results coming from T4, we had high hopes of getting the same results with T7. Our first attempts were unsuccessful but we believe we will be able to isolate the mutant T7 in the following weeks.


Daily log

Experiment Listing


Singlephage.JPG


August 17 - August 31


We ran into more problems attempting to purify the T7 phage. While we were at first able to get a clear band of phage using the cesium chloride gradient we were no longer able to achieve the same results. We focussed on creating a gradient that would allow the T7 phage to band once again but we did not have any luck in doing so.


Daily log

Experiment Listing



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Phage.JPG