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• iGEM 2013

Phage Purification July - August Notebook: Experiments

Phage Purification March-April May-June July-August September-October

7.31 EM Pictures I) Purpose To see if we have mutated, pure phage from the CsCl gradient and mutagenisis II) Expected Outcome Pictures of pure, intact phage, potentially mutated phage. III) Reagants Used Prepared Phage IV) Actual Procedure Gave prepared phage to TEM operator. We had four sample types: Phage from the 'petite' band in the CsCl gradient Phage from the 'large phage' band in the CsCl gradient V) Results Pictures of Phage Petite Phage Large Phage