Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period4/Exp/6.17PhagePreparation

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| colspan="3" | <font color="#333399" size="5" font face="Calibri"> '''Phage Purification May - June Notebook: Experiments'''</font>

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: [[Team:BYU_Provo/Phage_Purification|Overview]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Winterexp|March-April]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Springexp|May-June]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Summerexp|July-August]]

: [[Team:BYU Provo/Notebook/Phage_Purification/Fallexp|September-October]]

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<font size="5"> '''6.17 Phage Preparation''' </font>

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'''I) Purpose'''
: Grow phage to practice purification procedures.

'''II) Expected Outcome'''
: Viable stocks of both T4 and T7.

'''III) Reagants Used'''
: W3110 and BL21 freezer stocks
: LB
: T4 and T7 phage stocks


'''IV) Actual Procedure'''

: Infect 45 mL of LB with 2 loop-fulls of bacterial freezer stock and let sit at 37<sup>◦</sup> C overnight.
: Infect W3110 with 100 µL T4 and BL21 with 100 µL T7.
: Let both cultures sit overnight at 37<sup>◦</sup> C.

'''V) Results'''
: We created phage cultures to practice the purification procedure.

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