Team:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size
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* Experimental procedures are as follow: 0.5mL of E coli BL21 (OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated for 24 hours. | * Experimental procedures are as follow: 0.5mL of E coli BL21 (OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated for 24 hours. | ||
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+ | 2) Propagation and spot test of T1 (8.12-8.20) | ||
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+ | * Propagated T1 by infection 1mL E coli B and 4mL of LB with 10uL of stock phage. Complete clearage was observed in two days before purification via centrifuge (3000rpm for 5min) and chloroform (100uL of chloroform to 1mL of lysis). This stock of T1 is labeled T1P. | ||
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+ | * Dilution series -2 through -12 was performed for T1. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar. | ||
'''V) Results''' | '''V) Results''' | ||
1) Plating | 1) Plating | ||
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+ | * T1 plates had obvious contamination. Results need to be repeated. | ||
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+ | * T2 still formed larger than expected plaques. | ||
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Revision as of 21:47, 22 August 2013
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8.16 Modeling phage plaque size - Experiment One
I) Purpose
II) Expected Outcome
III) Reagents Used
IV) Procedure 1) Plating (8.16)
2) Propagation and spot test of T1 (8.12-8.20)
V) Results 1) Plating
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