http://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&feed=atom&action=historyTeam:BYU Provo/Notebook/SmallPhage/Summerexp/8.16 Modeling phage plaque size - Revision history2024-03-29T01:41:44ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=112567&oldid=prevXiuqili at 06:56, 10 September 20132013-09-10T06:56:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Propagated T1 by infection 1mL E coli B and 4mL of LB with 10uL of stock phage. Complete clearage was observed in two days before purification via centrifuge (3000rpm for 5min) and chloroform (100uL of chloroform to 1mL of lysis). This stock of T1 is labeled T1P.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Propagated T1 by infection 1mL E coli B and 4mL of LB with 10uL of stock phage. Complete clearage was observed in two days before purification via centrifuge (3000rpm for 5min) and chloroform (100uL of chloroform to 1mL of lysis). This stock of T1 is labeled T1P.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Dilution series -2 through -12 was performed for <del class="diffchange diffchange-inline">T1</del>. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Dilution series -2 through -12 was performed for <ins class="diffchange diffchange-inline">T1P</ins>. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3) Spot test for T2 from stock (8.20)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>3) Spot test for T2 from stock (8.20)</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4) Preliminary Titer (9.8)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4) Preliminary Titer (9.8)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Based on results from spot test for <del class="diffchange diffchange-inline">T1 </del>and T2, titer was performed for <del class="diffchange diffchange-inline">T1 </del>at -6, -7, and -8; and T2 at -2, -3, and -4. Specifically, 0.5mL of E coli B liquid culture overnight was infected with 20uL of respective phage sample. After 15 minutes of incubation, 5mL of x1 top agar (not precise) was added to each test tube, and the content was plated. The plates were incubated at 37 Celsius for 20 hours.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Based on results from spot test for <ins class="diffchange diffchange-inline">T1P </ins>and T2, titer was performed for <ins class="diffchange diffchange-inline">T1P </ins>at -6, -7, and -8; and T2 at -2, -3, and -4. Specifically, 0.5mL of E coli B liquid culture overnight was infected with 20uL of respective phage sample. After 15 minutes of incubation, 5mL of x1 top agar (not precise) was added to each test tube, and the content was plated. The plates were incubated at 37 Celsius for 20 hours<ins class="diffchange diffchange-inline">.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">5) Repeated plating for T1 and T2 (9.9)</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Preliminary titer revealed that T1P at -7 and T2 at -4 have adequate concentrations for plating. Plating procedures were the same as those recorded on 8.16. The only difference is the initial OD 600 reading of E coli B liquid culture overnight and subsequent dilutions: the average between two OD 600 readings was 0.513, but dilution was performed right after to adjust it to 0.5</ins>.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td></tr>
</table>Xiuqilihttp://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=111899&oldid=prevPerry721 at 20:01, 9 September 20132013-09-09T20:01:33Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* To see a statistical significance in plaque sizes between different phages, many plaques are needed. Thus 10 plates were used for T1, T2, T3, and T7. Because T4 form much smaller plaques, only 3 plates were used.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* To see a statistical significance in plaque sizes between different phages, many plaques are needed. Thus 10 plates were used for T1, T2, T3, and T7. Because T4 form much smaller plaques, only 3 plates were used.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Experimental procedures are as follow: 0.5mL of E coli <del class="diffchange diffchange-inline">BL21 </del>(OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated at 37 Celsius for 24 hours.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Experimental procedures are as follow: 0.5mL of E coli <ins class="diffchange diffchange-inline">B </ins>(OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated at 37 Celsius for 24 hours.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2) Propagation and spot test of T1 (8.12-8.20)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2) Propagation and spot test of T1 (8.12-8.20)</div></td></tr>
</table>Perry721http://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=109785&oldid=prevXiuqili at 00:17, 9 September 20132013-09-09T00:17:43Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4) Preliminary Titer (9.8)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>4) Preliminary Titer (9.8)</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline">Titer </del>was performed for T1 at -6, -7, and -8; and T2 at -2, -3, and -4. Specifically, 0.5mL of E coli B liquid culture overnight was infected with 20uL of respective phage sample. After 15 minutes of incubation, 5mL of x1 top agar (not precise) was added to each test tube, and the content was plated. The plates were incubated at 37 Celsius for 20 hours.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">Based on results from spot test for T1 and T2, titer </ins>was performed for T1 at -6, -7, and -8; and T2 at -2, -3, and -4. Specifically, 0.5mL of E coli B liquid culture overnight was infected with 20uL of respective phage sample. After 15 minutes of incubation, 5mL of x1 top agar (not precise) was added to each test tube, and the content was plated. The plates were incubated at 37 Celsius for 20 hours.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td></tr>
</table>Xiuqilihttp://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=109780&oldid=prevXiuqili at 00:16, 9 September 20132013-09-09T00:16:43Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* To see a statistical significance in plaque sizes between different phages, many plaques are needed. Thus 10 plates were used for T1, T2, T3, and T7. Because T4 form much smaller plaques, only 3 plates were used.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* To see a statistical significance in plaque sizes between different phages, many plaques are needed. Thus 10 plates were used for T1, T2, T3, and T7. Because T4 form much smaller plaques, only 3 plates were used.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Experimental procedures are as follow: 0.5mL of E coli BL21 (OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated for 24 hours.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Experimental procedures are as follow: 0.5mL of E coli BL21 (OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated <ins class="diffchange diffchange-inline">at 37 Celsius </ins>for 24 hours.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2) Propagation and spot test of T1 (8.12-8.20)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2) Propagation and spot test of T1 (8.12-8.20)</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Dilution series -2 through -7 was performed for T2 from stock. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Dilution series -2 through -7 was performed for T2 from stock. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">4) Preliminary Titer (9.8)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Titer was performed for T1 at -6, -7, and -8; and T2 at -2, -3, and -4. Specifically, 0.5mL of E coli B liquid culture overnight was infected with 20uL of respective phage sample. After 15 minutes of incubation, 5mL of x1 top agar (not precise) was added to each test tube, and the content was plated. The plates were incubated at 37 Celsius for 20 hours.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td></tr>
</table>Xiuqilihttp://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=105283&oldid=prevXiuqili at 04:56, 6 September 20132013-09-06T04:56:47Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>: <u> '''Small Phage''' </u></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><font size = "4"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>: <u> '''Small Phage''' </u<ins class="diffchange diffchange-inline">> </font</ins>></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]</div></td></tr>
</table>Xiuqilihttp://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=105282&oldid=prevXiuqili at 04:56, 6 September 20132013-09-06T04:56:30Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>: <u> '''Small Phage''' </u<del class="diffchange diffchange-inline">> </font</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>: <u> '''Small Phage''' </u></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]</div></td></tr>
</table>Xiuqilihttp://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=105281&oldid=prevXiuqili at 04:56, 6 September 20132013-09-06T04:56:16Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font color="#333399" size="3" font face="Calibri"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><font color="#333399" size="3" font face="Calibri"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>: <del class="diffchange diffchange-inline">[[Team:BYU_Provo</del>/<del class="diffchange diffchange-inline">Small_Phage|Overview]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>: <ins class="diffchange diffchange-inline"><u> '''Small Phage''' <</ins>/<ins class="diffchange diffchange-inline">u> </font></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>: [[Team:BYU Provo/Notebook/SmallPhage/Winterexp|March-April]]</div></td></tr>
</table>Xiuqilihttp://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=97985&oldid=prevXiuqili at 21:21, 1 September 20132013-09-01T21:21:35Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 21:21, 1 September 2013</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Dilution series -2 through -12 was performed for T1. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Dilution series -2 through -12 was performed for T1. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">3) Spot test for T2 from stock (8.20)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Dilution series -2 through -7 was performed for T2 from stock. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T1</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-2</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">18 w/ overlap</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|<ins class="diffchange diffchange-inline">contamination</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-3</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>| </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">0</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
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<td colspan="2" class="diff-lineno">Line 100:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T2</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T2</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-4</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">22 w/ overlap</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-5</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">6</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 105:</td>
<td colspan="2" class="diff-lineno">Line 109:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T3</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T3</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-5</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">33 w/ overlap</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-6</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">7</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
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<td colspan="2" class="diff-lineno">Line 118:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T4</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T4</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-6</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>| </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">a lot w/ overlap</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-7</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">47</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 123:</td>
<td colspan="2" class="diff-lineno">Line 127:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T7</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|T7</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-4</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>| </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">a lot w/ overlap</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| <del class="diffchange diffchange-inline">-5</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|<del class="diffchange diffchange-inline">18</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 133:</td>
<td colspan="2" class="diff-lineno">Line 137:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></center></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2) T1 and T2 spot test</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* T1 showed plaques up to -8 after propagation, while -2 showed plaques up to -4.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Xiuqilihttp://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=84068&oldid=prevXiuqili at 21:47, 22 August 20132013-08-22T21:47:09Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:47, 22 August 2013</td>
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<td colspan="2" class="diff-lineno">Line 58:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Experimental procedures are as follow: 0.5mL of E coli BL21 (OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated for 24 hours.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Experimental procedures are as follow: 0.5mL of E coli BL21 (OD 0.5, adjusted after initial measurement of 0.558) was infected with 20uL of respective phage dilution samples for 10 minutes. 5mL x1 top agar was then added to each test tube, and the content was plated. The plates were incubated for 24 hours.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">2) Propagation and spot test of T1 (8.12-8.20)</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Propagated T1 by infection 1mL E coli B and 4mL of LB with 10uL of stock phage. Complete clearage was observed in two days before purification via centrifuge (3000rpm for 5min) and chloroform (100uL of chloroform to 1mL of lysis). This stock of T1 is labeled T1P.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* Dilution series -2 through -12 was performed for T1. 5uL of each dilution sample was spotted onto plates overlaid with 0.5mL of E coli B and 5mL of x1 agar.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''V) Results'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1) Plating</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1) Plating</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* T1 plates had obvious contamination. Results need to be repeated.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">* T2 still formed larger than expected plaques.</ins></div></td></tr>
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</table>Xiuqilihttp://2013.igem.org/wiki/index.php?title=Team:BYU_Provo/Notebook/SmallPhage/Summerexp/8.16_Modeling_phage_plaque_size&diff=76699&oldid=prevXiuqili at 21:41, 17 August 20132013-08-17T21:41:48Z<p></p>
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