Team:BostonU/QS

From 2013.igem.org

(Difference between revisions)
Line 3: Line 3:
<html>
<html>
<br>
<br>
-
<h1>Quorum Sensing</h1>
+
<h2>Quorum Sensing</h2>
<h7>
<h7>
<p>Quorum sensing is a system comprising of stimuli and responses associated with population density.  Bacteria utilize quorum sensing to coordinate gene expression.  The bacteria produce and secrete signaling molecules.  These bacteria have a receptor that detects the signaling molecule.  When the signaling molecule binds to the receptor, it activates a reporter.</p>
<p>Quorum sensing is a system comprising of stimuli and responses associated with population density.  Bacteria utilize quorum sensing to coordinate gene expression.  The bacteria produce and secrete signaling molecules.  These bacteria have a receptor that detects the signaling molecule.  When the signaling molecule binds to the receptor, it activates a reporter.</p>

Revision as of 19:54, 17 August 2013


Quorum Sensing

Quorum sensing is a system comprising of stimuli and responses associated with population density. Bacteria utilize quorum sensing to coordinate gene expression. The bacteria produce and secrete signaling molecules. These bacteria have a receptor that detects the signaling molecule. When the signaling molecule binds to the receptor, it activates a reporter.

INSERT IMAGE HERE

A LuxIR-type quorum sensing system has been detected in Chromobacterium violaceum (Stauff et al., 2011). The goal is to introduce a new LuxR/I-like quorum sensing system to synthetic biology wia MoClo. Chromobacterium violaceum, a gram-negative bacteria found in flora from water and soil in tropical and subtropical regions, uses the CviR/I system. The CviR/I system is homologous to the LuxR/I system. This can be done by cloning and characterizing CviR/I and pVioA, a promoter with CviR binding site) into E.coli.


References

[1] Stauff, D.L., and Bassler, B.L. (2011) Quorum Sensing in Chromobacterium violaceum: DNA Recognition and Gene Regulation by the CviR Receptor. Journal of Bacteriology 193(15):3871-3878. DOI: 10.1128/JB.05125-11