Team:BostonU/Results

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<h2>Results Summary</h2>
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<h7><p>Below is a brief summary of our results from this summer. Each statement has a link shown in </h7><h8>RED</h8><h7> to the page(s) containing more information and detailed results. </h7> </p>
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<br><p>This summer, we created 63 new Level 0 Parts (32 prom, 12 5'UTR, 16 CDS, and 3 terminators), 14 new Destination Vectors, and 86 new Level 1 Transcriptional Units to the <a href="https://2013.igem.org/Team:BostonU/Parts">MoClo Library</a></p>
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<p>We have also updated the <a href="https://2013.igem.org/Team:BostonU/MoClo">MoClo Kit</a> to include 90 Level 0 Basic Parts and 21 Destination Vectors so teams next year can create devices containing up to 4 transcriptional units</p>
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<p  style="text-align:center;"><a href="https://2013.igem.org/Team:BostonU"><img src="https://static.igem.org/mediawiki/2013/2/2c/Marsh.png" width="800px" length="200px"></a></p>
 
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<li><a href="#">Our Team</a>
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<li><a href="https://2013.igem.org/Team:BostonU/Team">Team</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Social">Summer Fun</a></li>
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<li><a href="#">Project</a>
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<li><a href="https://2013.igem.org/Team:BostonU/Project_Overview">Project Overview and Abstract</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/MoCloChara">Introduction to MoClo and Characterization</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/QS">Quorum Sensing</a></li>
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<tr><td><img src="https://static.igem.org/mediawiki/2013/d/d8/Char_data_BU.gif" width="200px"></td>
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                        <li><a href="https://2013.igem.org/Team:BostonU/HK">Histidine Kinase</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/ML">MoClo Library</a></li>
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<br><p>Using a state of the art BD LSRFortessa at the Center of Synthetic Biology here at Boston University, we have successfully <a href="https://2013.igem.org/Team:BostonU/Data">characterized</a> 64 Level 1 Transcriptional Units and 2 Level 2 Devices</p>
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                        <li><a href="https://2013.igem.org/Team:BostonU/DataSheet">Data Sheets App</a></li> 
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<p>From this work, we will draft a proposal for a standardized flow cytometry protocol for future teams to utilize when testing devices containing fluorescent proteins in <i>E. coli</i></p>
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<li><a href="https://2013.igem.org/Team:BostonU/Methodology ">Methodology Overview</a></li>  
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<li><a href="https://2013.igem.org/Team:BostonU/Results ">Results Summary</a></li>
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<li><a href="#">Achievements</a>
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<li><a href="https://2013.igem.org/Team:BostonU/Data">Data Collected</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Parts">Parts Submitted</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/MoClo">MoClo Kit</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Gold">Medal Fulfillment</a></li>  
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<li><a href="#">Notebook</a>
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<li><a href="https://2013.igem.org/Team:BostonU/Protocols">Protocols</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Clotho">Clotho and Eugene</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/NotebookQS">Quorum Sensing Notebook</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookHK">Histidine Kinase Notebook</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/NotebookML">MoClo Library Notebook</a></li>
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                        <li><a href="https://2013.igem.org/Team:BostonU/TroubleShooting">Trouble Shooting</a></li>
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<li><a href="#">Considerations</a>  
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<li><a href="https://2013.igem.org/Team:BostonU/NEGEM">New England iGEM Regional Meeting</a></li>
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<li><a href="https://2013.igem.org/Team:BostonU/Human Practices">Human Practices</a></li>
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<br><p>Working closely with the <a href="https://2013.igem.org/Team:Purdue">Purdue Biomakers</a> team, we are designing a <a href="https://2013.igem.org/Team:BostonU/DataSheet">datasheet</a> that will allow users to more easily share information and data within the iGEM community and beyond</p>
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<li><a href="https://2013.igem.org/Team:BostonU/Safety">Safety</a></li>
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<p>We have begun implementing a Java-based web tool to generate these data sheets</p>
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<li><a href="#">Acknowledgements</a>  
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                    <li><a href="https://2013.igem.org/Team:BostonU/Collaborations">Collaborations</a></li>
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                    <li><a href="https://2013.igem.org/Team:BostonU/Acknowledgements">Acknowledgements</a></li>
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Latest revision as of 03:29, 28 September 2013



Results Summary

Below is a brief summary of our results from this summer. Each statement has a link shown in RED to the page(s) containing more information and detailed results.




      This summer, we created 63 new Level 0 Parts (32 prom, 12 5'UTR, 16 CDS, and 3 terminators), 14 new Destination Vectors, and 86 new Level 1 Transcriptional Units to the MoClo Library

      We have also updated the MoClo Kit to include 90 Level 0 Basic Parts and 21 Destination Vectors so teams next year can create devices containing up to 4 transcriptional units



      Using a state of the art BD LSRFortessa at the Center of Synthetic Biology here at Boston University, we have successfully characterized 64 Level 1 Transcriptional Units and 2 Level 2 Devices

      From this work, we will draft a proposal for a standardized flow cytometry protocol for future teams to utilize when testing devices containing fluorescent proteins in E. coli



      Working closely with the Purdue Biomakers team, we are designing a datasheet that will allow users to more easily share information and data within the iGEM community and beyond

      We have begun implementing a Java-based web tool to generate these data sheets