Team:Braunschweig/Notebook

From 2013.igem.org

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Today we also observed our cells containing the chromoprotein expression cassette under the fluorescence microscope.
Today we also observed our cells containing the chromoprotein expression cassette under the fluorescence microscope.
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<img alt="Chromoprotein expression" src="https://static.igem.org/mediawiki/2013/0/09/Braunschweig_Lab_Week13_microscope.jpg" height="200px" align="left" vspace="0" hspace="10" style="margin-right:5px"/>
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<img alt="Chromoprotein expression" src="https://static.igem.org/mediawiki/2013/0/09/Braunschweig_Lab_Week13_microscope.jpg" height="220px" align="left" vspace="0" hspace="10" style="margin-right:5px"/>
During the day we noticed that the 2xYT medium used for the pre-cultures yesterday showed contamination. Thus, there is a high possibility  of contamination in the pre-culture as well as in the main culture. Therefore we decided to repeat the experiment. New pre-cultures of the three chromoprotein expression cassettes where inoculated in 50 ml 2xYT containing chloramphenicol directly from -80°C.<br>
During the day we noticed that the 2xYT medium used for the pre-cultures yesterday showed contamination. Thus, there is a high possibility  of contamination in the pre-culture as well as in the main culture. Therefore we decided to repeat the experiment. New pre-cultures of the three chromoprotein expression cassettes where inoculated in 50 ml 2xYT containing chloramphenicol directly from -80°C.<br>

Revision as of 23:12, 4 October 2013

Labjournal

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Braunschweig Labbook This is the documentation of our lab work. Achievements of each week are summerized followed by a daily discription of our experiments.

An overview on how we approached this project can be found under Approach. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our
Attributions section for efforts beyond the lab work.


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