Team:Braunschweig/Notebook

From 2013.igem.org

(Difference between revisions)
Line 754: Line 754:
<p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px">
<p style="margin-left:5px; margin-right:5px; margin-bottom:0px; margin-top:0px">
<b>Investigators: Kevin, Anna, Melanie</b><br>
<b>Investigators: Kevin, Anna, Melanie</b><br>
-
<img alt="August 2" src="https://static.igem.org/mediawiki/2013/c/cb/Braunschweig_Lab_Journal_August_2.png" width="250"  vspace="20" align="right"/>In order to test the success of the last days’ cloning experiment we conducted a colony PCR. Unfortunately all clones of both our supposed final Prhl constructs were negative. Therefore the PCR was repeated later with new colonies.  
+
<img alt="August 2" src="https://static.igem.org/mediawiki/2013/c/cb/Braunschweig_Lab_Journal_August_2.png" width="250"  vspace="20" align="left" style="margin-right:5px"/>In order to test the success of the last days’ cloning experiment we conducted a colony PCR. Unfortunately all clones of both our supposed final Prhl constructs were negative. Therefore the PCR was repeated later with new colonies.  
The N-buturyl-HSL synthase RhlI with amilGFP reporter cassette (B0032-C0070-B0015-J23100-B0032-K592010-B0015) and the eCFP cassette (J23100-E0420) showed both clones bearing the correct construct.<br>
The N-buturyl-HSL synthase RhlI with amilGFP reporter cassette (B0032-C0070-B0015-J23100-B0032-K592010-B0015) and the eCFP cassette (J23100-E0420) showed both clones bearing the correct construct.<br>
We also conducted a test in 5 mL 2xYT liquid cultures with different concentrations of ampicillin and autoinducers to test if our new constructs were leaky. Cells bearing the inducible ampicillin resistance and LasR transcription factor (B0015-K091117-B0032-ampR-B0015-J23100-B0032-C0079) were tested with ampicillin supplements between 1 and 8 µg/mL. We expected no growth as the ampicillin resistance was not induced.<br>
We also conducted a test in 5 mL 2xYT liquid cultures with different concentrations of ampicillin and autoinducers to test if our new constructs were leaky. Cells bearing the inducible ampicillin resistance and LasR transcription factor (B0015-K091117-B0032-ampR-B0015-J23100-B0032-C0079) were tested with ampicillin supplements between 1 and 8 µg/mL. We expected no growth as the ampicillin resistance was not induced.<br>

Revision as of 01:27, 5 October 2013

Labjournal

linie rot 8pix hoch

Braunschweig Labbook This is the documentation of our lab work. Achievements of each week are summerized followed by a daily discription of our experiments.

An overview on how we approached this project can be found under Approach. For detailed protocols of certain procedures please refer to Protocols. Attributions are given for each day, however please check our
Attributions section for efforts beyond the lab work.


grey line

Our sponsors

linie rot 8pix hoch