Team:Braunschweig/Protocols

From 2013.igem.org

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<div id="Recipes" class="menuSection">
     <h2><a href="#Recipes">Recipes for Solutions and Media</a></h2>
     <h2><a href="#Recipes">Recipes for Solutions and Media</a></h2>
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     <p><p style=" margin-left:5px; margin-right:5px; margin-bottom:0px;">Coming soon.</p>
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<b>2x YT-medium</b><br>
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16 g Bacto tryptone<br>
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10 g Bacto yeast extract<br>
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5 g NaCl<br>
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were dissolved in 1 L DI water, the pH was adjusted to 7.0.<br>
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For solid medium 12 g of Bacto agar (per Liter) was added.<br><br>
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<b>50x TAE Buffer stock solution</b><br>
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242 g 2M Tris base<br>
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51,1 mL glacial acetic acid (17,4M)<br>
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200 mL 100mM EDTA (pH 8)<br>
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were dissolved in 1L DI water.<br>
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<b>Loading buffer for gel electrophoresis</b><br>
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A 0.2M EDTA and 0.05% (w/v) orange G or bromphenoleblue solution was prepared in 50% glycerine. <br><br>
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<b>Ampicillin stock solution</b><br>
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10 g Ampicillin sodium salt was dissolved in 100 mL DI water, sterile filtered, aliquoted and stored at -20 °C.<br><br>
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<b>Chloramphenicol stock solution</b><br>
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3.4 g Chloramphenicol was dissolved in 100 mL Ethanol, sterile filtered, aliquoted and stored at -20 °C.<br><br>
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<b>Clavulanic acid</b><br>
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1 g Clavulanic acid was dissolved in 100 mL Ethanol, sterile filtered aliqoted and stored at -20 °C.
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Revision as of 18:37, 25 September 2013

Protocols

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In this section you will find detailed protocols of experimental procedures.

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