Team:Braunschweig/Protocols

From 2013.igem.org

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     <h2><a href="#PCR">PCR</a></h2>
     <h2><a href="#PCR">PCR</a></h2>
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     <p><p style=" margin-left:5px; margin-right:5px; margin-bottom:0px;">Colony PCR, Phusion PCR coming soon</p>
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<b>Colony PCR</b><br>
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<img alt="ColonyPCR" src="https://static.igem.org/mediawiki/2013/3/3d/Braunschweig_Protocols_Colony_PCR_new.png" width="600" align="right" vspace="0" hspace="20"/>
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After successful transformation single colonies were picked and analyzed by colony PCR. Therefor each colony was picked from the original plate with a pipette tip and dipped into the PCR-mix. Afterwards the pipette tip was used to inoculate a second agarplate. PCR fragments were analyzed via gelectrophoresis.<br><br>
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<b>DNA amplification via PCR with NEB Phusion® High-Fidelity DNA Polymerase </b><br>
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<img alt="PhusionPCR" src="https://static.igem.org/mediawiki/2013/9/99/Braunschweig_Protocols_Phusion_PCR.png" width="600" align="left" vspace="0" hspace="20"/>
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Revision as of 23:52, 28 September 2013

Protocols

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In this section you will find detailed protocols of experimental procedures.

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