Team:Braunschweig/Protocols

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     <h2><a href="#Agardiffusiontest">Agar Diffusion Test</a></h2>
     <h2><a href="#Agardiffusiontest">Agar Diffusion Test</a></h2>
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     <p><p style=" margin-left:5px; margin-right:5px; margin-bottom:5px;">Overnight cultures of <i>E. Coli</i> JM109 carrying BBa_K1073034 and <i>E. Coli</i> TOP10F’ carrying BBa_K1073035 were grown on 2x YT medium containing Ampicillin and the respective autoinducer. The next day the cells were harvested by centrifugation at 4000 rpm for 10 min and subsequently resuspended in fresh 2xYT medium. The obtained cultures were then spread out on Ampicillin containing agar plates. A filter paper impregnated with the respective synthetic autoinducer solution was laid on top of the freshly spread agar plate. Afterwards the plates were incubated at 37 °C for 3-4 h and pictures were taken to document the circles of bacterial growth around the filter.
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     <p><p style=" margin-left:5px; margin-right:5px; margin-bottom:5px;">Overnight cultures of <i>E. Coli</i> JM109 carrying BBa_K1073034 and <i>E. Coli</i> TOP10F’ carrying BBa_K1073035 were grown on 2x YT medium containing Ampicillin and the respective autoinducer. The next day the cells were harvested by centrifugation at 4000 rpm for 10 min and subsequently resuspended in fresh 2xYT medium. The obtained cultures were then spread out on Ampicillin containing agar plates. A filter paper impregnated with the respective synthetic autoinducer solution was laid on top of the freshly spread agar plate. Afterwards the plates were incubated at 37 °C for 3-4 h and pictures were taken to document the circles of bacterial growth around the filter.<br>
This experiment was also conducted with the supernatant of the respectively other strain as the two strains cross induce each other.
This experiment was also conducted with the supernatant of the respectively other strain as the two strains cross induce each other.
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Revision as of 23:18, 2 October 2013

Protocols

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In this section you will find detailed protocols of experimental procedures.

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