Team:Braunschweig/Protocols

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<div id="Fluorescenceimaging" class="menuSection">
<div id="Fluorescenceimaging" class="menuSection">
     <h2><a href="#Fluorescenceimaging">Imaging of Cells producing Chromoproteins</a></h2>
     <h2><a href="#Fluorescenceimaging">Imaging of Cells producing Chromoproteins</a></h2>
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     <p><p style=" margin-left:5px; margin-right:5px; margin-bottom:5px;">Cells were grown in 100 ml 2xYT medium containing ampicillin and 10 µM N-buturyl homoserine lactone (P<sub>Rhl</sub>) or N-3-oxododecanoyl homoserine lactone (P<sub>Las</sub>) at 37°C and 250 rpm over night. The medium was inoculated directly from -80°C glycerol cell stock.  
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     <p><p style=" margin-left:5px; margin-right:5px; margin-bottom:5px;">Cells were grown in 100 ml 2xYT medium containing ampicillin and 10 µM N-buturyl homoserine lactone (Prhl) or N-3-oxododecanoyl homoserine lactone (Plas) at 37°C and 250 rpm over night. The medium was inoculated directly from -80°C glycerol cell stock.  
Cells were diluted with fresh 2xYT medium containing ampicillin and suspension was placed between to agar layers containing ampicllin as well.<br>  
Cells were diluted with fresh 2xYT medium containing ampicillin and suspension was placed between to agar layers containing ampicllin as well.<br>  
Cell growth and division were observed for 3 hours by exciting chromoproteins every five minutes at specific wavelength (see absorption spectra) using YFP und mCherry filters.  
Cell growth and division were observed for 3 hours by exciting chromoproteins every five minutes at specific wavelength (see absorption spectra) using YFP und mCherry filters.  

Revision as of 01:41, 5 October 2013

Protocols

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Braunschweig Labbook

In this section you will find detailed protocols of experimental procedures.

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