Team:British Columbia/Notebook/CRISPR

From 2013.igem.org

Revision as of 01:54, 23 September 2013 by AnnaMüller (Talk | contribs)

iGEM Home

Contents

Team CRISPR

July 3

List of BioBricks that are/will be on the way:

Cas9:

  • Cas9 - Joe and Anna
  • pTet -
  • const. promoter + Cas9 - Joe
  • promoter + Cas9 + Leader + R + spacer + R (on Kan)
  • L + R + spacer + R

Target:

  • tracerRNA
  • repeat + spacer + repeat - Vanins*2 + underlings
  • leader - Ray, Fisal, Dan

Decoy:

  • PAM + spacer (on Amp)


July 10th

Experimenter:Anna Müller

Aim:PCR the cas9 gene from Streptococcus thermophilus cells.

Results: unsuccesful no bands were seen on the agerose gel.


September 22nd

Experimenter: Anna Müller

Aim: Digest T4, T7 spacer sequence with PAM site(decoy construct) with EcoRI and SpeI to later on ligate into plasmid with ampicillin resistance.

Results: will follow.


Aim: Digest PsBIA3 plasmid with ampicillin resistance with EcoRI and SpeI to construct the decoy plasmides.

Results: will follow.