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Team:Buenos Aires/ model
From 2013.igem.org
Deterministic Model
Introduction
Our objective was to assemble a system that could respond to different concentrations of a specific contaminant in water. More specifically, the idea was to produce different amounts of a coloured pigment depending on the concentration of the contaminant present in water.
As mention all over this wiki, we worked with Arsenic knowing that an Ars operon exist in nature and more important, it exists as a biobrick in IGEM.
Got Ideas?
For our biosensor we came up with three different designs each one motivated on improving the flaws detected while analysing the previous ones using mathematical models as our main tools. we describe briefly the first two designs which were later rejected to emphasize the importance of mathematical model to gain insight on how our system works and as a feasibility study.
For the first idea we keep it simple:
Unfortunately
As it is shown in the figure below, in the presence of arsenite (1000 ppb) a typical transcriptional induction is observed over time. There is a lag of 3 hours in the mRFP produciton after adding arsenite.
