"
Team:Buenos Aires/ res dmrfp
From 2013.igem.org
(Difference between revisions)
(→Hibrid promoter induction) |
(→GFP production in th presence of different arsenite concentrations) |
||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
<div id="external"> | <div id="external"> | ||
| - | = | + | = Hibrid prmoter= |
'''Protocol''' | '''Protocol''' | ||
| - | Several cultures carrying a plasmid that encodes GFP under an arsenite | + | Several cultures carrying a plasmid that encodes GFP under an arsenite sensitivnnnnnnnnnnnne promoter (Bba_K1106004) were grown overnight with different arsenite concentration '''(0, 10,..ppb)''' |
Fluorescence was measured with a fluorimeter at '''x nm''' exiitation wavelength. All theese data was normalized by the culture density measured by OD. | Fluorescence was measured with a fluorimeter at '''x nm''' exiitation wavelength. All theese data was normalized by the culture density measured by OD. | ||
Latest revision as of 16:17, 27 September 2013
Hibrid prmoter
Protocol
Several cultures carrying a plasmid that encodes GFP under an arsenite sensitivnnnnnnnnnnnne promoter (Bba_K1106004) were grown overnight with different arsenite concentration (0, 10,..ppb) Fluorescence was measured with a fluorimeter at x nm exiitation wavelength. All theese data was normalized by the culture density measured by OD.
Results
