GFP production in th presence of different arsenite concentrations

Protocol

Several cultures carrying a plasmid that encodes GFP under an arsenite sensitive promoter (Bba_K1106004) were grown overnight with different arsenite concentration (0, 10,..ppb) Fluorescence was measured with a fluorimeter at x nm exiitation wavelength. All theese data was normalized by the culture density measured by OD.

Results