Team:Buenos Aires/ res qrfp

From 2013.igem.org

(Difference between revisions)

Latest revision as of 23:39, 22 September 2013

Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) where grown at 30°C until they reach OD=0.4 (600nm). At this point arsenite was added (10ppb and 1000ppb of arsenite final concentration in each other) fluorescence was measured every 30 minutes for 8 hours with a fluorimeter at 584 nm excitation peak and 607 nm emission peak. All these data was normalized by the culture density measured by OD.

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-Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 | Bba_K1106003]) where grown at 30°C until they reach OD=0.4 (600nm). At this point arsenite was added (10ppb and 1000ppb of arsenite final concentration in each other) fluorescence was measured every 30 minutes for 8 hours with a fluorimeter at 584 nm excitation peak and 607 nm emission peak. All these data was normalized by the culture density measured by OD.
+Two 100 ml culture of EColi DH5alfa carrying a plasmid with a mRFP generator under arsenite sensitive promoter ([http://parts.igem.org/Part:BBa_K1106003 Bba_K1106003]) where grown at 30°C until they reach OD=0.4 (600nm). At this point arsenite was added (10ppb and 1000ppb of arsenite final concentration in each other) fluorescence was measured every 30 minutes for 8 hours with a fluorimeter at 584 nm excitation peak and 607 nm emission peak. All these data was normalized by the culture density measured by OD.
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