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Team:CU-Boulder/Notebook/Experiments
From 2013.igem.org
- Experiment 1.1 - Chemical Transformation Efficiency
- This experiment was designed to test several parameters of our transformation protocol to determine ways of increasing our transformation efficiency.
- Experiment 2.1 - Competent Cell Efficiency
- In this experiment we determined the relative efficiency of three different batches of competent cells; one prepared using a protocol supplied by our advisor, Alex, and the other two prepared according to the iGEM protocol.
- Experiment 2.2 - Transformation Recovery Period
- We tested the effect of varying the recovery period of our transformation protocol on transformation efficiency.
- Experiment 2.3 - Transformation Recovery Medium
- In this experiment we tried using different types and volumes of growth media during the recovery phase of our transformation protocol to see which worked the best.
- Experiment 3.1 - Promega Miniprep Protocol
- Several parameter of the Promega miniprep protocol were varied in this experiment to investigate their effect on DNA recovery.
- Experiment 5.1 - Calculating Competent Cell Efficiency
- We calculated the efficiency of our competent cells using iGEM's transformation efficiency kit.
- Experiment 6.1 - Competent Cell Efficiency
- This experiment was conducted to compare the relative efficiency of a new batch of competent cells to our original supply.
- Experiment 6.2 - Gel Separation of Proteins Methods
- In this experiment we tested different ways to separate RFP with an agarose gel.
- Experiment 10.1 - Preliminary Homemade Miniprep Buffer Experiment
- This experiment tests the effectiveness of each of our homemade miniprep buffers individually within the Qiagen miniprep system.
- Experiment 11.1 - Mini-column Recycling Protocol
- This experiment was conducted to test whether our mini-column recycling protocol was truly effective at removing residual DNA from used miniprep mini-columns.
- Experiment 11.2 - Homemade vs. Qiagen Miniprep Buffers
- Here we tested our homemade miniprep buffers against the commercial Qiagen buffers as a complete set.
- Experiment 11.3 - Mini-column Recycling Protocol Pt. II
- This experiment was designed to compare the effectiveness of recycled mini-columns to new mini-columns.
- Experiment 12.1 - Calculating Competent Cell Efficiency
- Here we tested the efficiency of our most recent batch of competent cells using iGEM's tranformation efficiency kit.
- Experiment 14.1 - Gel Separation of Proteins
- In this experiment we tested how effective separating AmilCP from RFP was on an agarose gel.
- Experiment 15.1 - Protein Purification Using RTX
- This experiment tested the efficacy of RTX as a protein purification method.
