Team:CU-Boulder/Project/Attribution

We could like to thank the following people for all their hard work and help.

We would like to thank the following labs for donating lab space and usage of equipment for these experiments.

• Copley Lab
• Garcia Lab
• Dowell Lab

Thanks to all of our advisors

These people did a lot to show us how to use the equipment safely, how to perform the various protocols we needed, where to look for information and all the support services like ordering DNA sequences from IDT.

• Faculty Advisor:Robin Dowell D.Sc
• Graduate Student Advisor: Joe Rokicki
• Graduate Student Advisor: Mike Brasino
• Graduate Student Advisor: Rebecca Zabinsky
• Graduate Student Advisor: Tim Read
• Graduate Student Advisor: Jennifer Avena
• Graduate Student Advisor: Alex Stemm-Wolf
• Graduate Student Advisor: Thomas Reynolds
• Graduate Student Advisor: Isaac Godfroy

Thanks for our sponsors

We would like to thank all of our sponsors for the supplies, software and money to made this project possible

• Sponsors

DNA order optimization and ordering

Most of our parts had to be created by IDT in 500 bp blocks that were then gibson assembled.

• Joe Rokicki: Thanks for ordering the gblocks, designing how the blocks would be assembled along with head and tail sequences and doing a huge amount of work in checking the accuracy of the order and labeling all the features.
• William Heymann: For writing the MATLAB code used to figure out the sequences that would go in each block to make sure that each piece spanned as few pieces as possible and for codon optimizing all the sequences.

In order to create this order we used the following pieces of software and we wish to thank the creators

• MATLAB: The global optimizer is a truly amazing tool
• Gene Designer: This tool proved to be very useful for codon optimization and the company was very helpful
• UGENE: We used this program to identify the features in all of our sequences and to later verify all the parts we sent off for sequencing.

Researching and finding the correct sequences to order

This is something that all of the students and faculty advisors worked on pretty equally. We found these sequences before we started any of the lab work over the summer.

Thanks to the following people for checking the DNA sequence and AA sequences (after they where codon optimized) in order to ensure order accuracy.

• Phil Jensen
• William Heymann
• JF Lalonde
• Mike Brasino

Keeping track of all of our parts

• Phil Jensen: Thanks for setting up the spreadsheets for keeping track of all of our parts, where they were and what resistance they were on.

Write up experiments and protocols

• Phil Jensen
• William Heymann
• JF Lalonde

Work with AmilCP: Expression cassette, freiburg version, characterization, analysis, etc

• William Heymann
• Phil Jensen
• Graduate Student Advisor: Joe Rokicki
• Graduate Student Advisor: Alex Stemm-Wolf

Gel Separation for DNA and Protein

• William Heymann
• Phil Jensen
• Graduate Student Advisor: Joe Rokicki
• Graduate Student Advisor: Alex Stemm-Wolf

Work with RTX: freiburg version, characterization, analysis, etc

• JF Lalonde
• Graduate Student Advisor: Joe Rokicki

EcoRI

• Josephina Hendrix

T-Shirt Design

• Raleigh Ems

Website design

• William Heymann

I am sure there are some things that have been forgotten so thanks to everyone that has helped and worked so hard on this project.