Team:Calgary/Project/Achievements

From 2013.igem.org

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<h2>For our <span class="blue">Sensor</span> we...</h2>
<h2>For our <span class="blue">Sensor</span> we...</h2>
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<ul><li>Amended two TALEs from the registry and resubmitted these parts. We optimized the TALEs such that they can be expressed in <i> E. coli </i> by removing the eukaryotic kozak sequence present in the original parts, codon optimizing it for <i> E. coli</i> and added a his-6 tag such that this protein can be purified using affinity chromatography.</li>
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<ul>
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<li> We submitted two novel reporters: beta-lactamase and ferritin. We showed that these enzymes </li>
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<li>Designed a modular platform system which allows the interchangeability of a DNA binding protein, creating the potential for multiple different DNA sensors to be developed from the framework we have submitted</li>
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<li> We characterized beta-lactamase as a colorimetric reporter using phenol red. We also showed that beta </li>
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<li>Designed a DNA binding TALE which binds specifically to regions of the shiga toxin gene stx2 which are conserved amongst a wide variety of pathogenic E. coli</li>
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 +
<li>Submitted parts for an engineered ferritin gene, a biological nanoparticle capable of both scaffolding and stabilizing proteins, as well as carrying out catalytic reactions to act as a reporter</li>
 +
 
 +
<li>Designed, constructed, expressed, and successfully purified proteins for both the DNA sensing elements of our prototype as well as the scaffolding and reporter components of the system</li>
 +
 
 +
<li>Characterized the ability and kinetic properties of ferritin protein to act as a reporter</li>
 +
 
 +
<li>Characterized the ability for our purified ferritin produced from our construct to act as a reporter in solution as well as on a nitrocellulose membrane</li>
 +
<li>Created multiple versions of a prototype of our final system</li>
 +
 
 +
<li>Performed modelling to inform both the quantitative imputs and construction of our system, as well as visual modelling in order to communicate how our system works in addition to informing the wetlab team of</li>
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<li>3D printed components of our system in order to better understand how our parts would look at a molecular basis</li>
</ul>
</ul>
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<h2>For our <span class="orange">Human Practices</span> we...</h2>
<ul>
<ul>
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<h2>For our <span class="orange">Human Practices</span> we...</h2>
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<li>Created a dialogue with multiple industry experts and potential users in the field where our product could be applied</li>
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<h2>For our <b>Informed Design</b> we...</h2>
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<li>Visited all steps of the processing chain in order to better understand the culture and practices of the industry</li>
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<li> We used Informed Design: We designed our project with the cattle industries culture and needs first and foremost. </li>  
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<li>Used discussions with our stakeholders to inform the design of our final system from inception towards completion so that it puts industry culture and needs first and foremost</li>
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<li> Our project began with extensive consultation with key cattle industry stakeholders who explained the industries culture and how our technology could best fit into this it. We spoke with a rancher (Bob Church), feedlot operator (Chinook Feeders), a veterinarian (Feedlot Management Services), a meat processor (Cargill), and vaccine producer (Bioniche). </li>  
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<li>Designed our system with considerations towards safety, within our laboratory and in the implementation of the final prototype</li>
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<li> We continued to consult with these industry stakeholders throughout the development of our FerriTALE system to ensure our technology would continue to meet the needs of the industry.  Their industrial experience helped us to at many points during the summer redesign and pivot our system to better fit within the industry.</li>  
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<li>Thought extensively about the implications of our system in terms of the profound impact on community health and economics if false positives and negatives were to occur, and have included both  positive and negative controls in our system to mitigate these implications</li>
</ul>
</ul>
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<h2>For our <span class="yellow">Collaboration</span> we...</h2>
<ul>
<ul>
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<li>Worked with Paris-Bettencourt in defining biosensors for the iGEM community</li>
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<li>Worked in Consort Alberta over the summer in order to develop a system for detecting xylene contamination in soils</li>
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 +
<li>Reviewed all previous iGEM projects in order to categorize which developed biosensors</li>
 +
 +
<li>Consolidated this information into an easy to use database (SensiGEM) in order to allow for the community to search all previous sensors to gain useful insight and find useful parts when developing future projects</li>
 +
 +
</ul>
<h2>For our <span class="yellow">Collaboration</span> we...</h2>
<h2>For our <span class="yellow">Collaboration</span> we...</h2>
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<li> We collaborated with the Paris Bettencourt iGEM Team in developing a database of biosensors made in the iGEM competitions.</li>
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<ul>
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 +
<li>Continued our collaboration with our local science centre, Telus Spark, in order to educate our community about synthetic biology and create activities which would bring the science to a level which would allow for education through something fun</li>
 +
 
 +
<li>Brought basic principles of synthetic biology to our city as a whole through our participation in running activites at our cities Beakernight event</li>
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 +
<li>Gave the world an insight into the daily life of an iGEMmer as well as sharing synthetic biology through blog posts written for Genome Alberta</li>
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<h2>For our <b>Parts</b> we...</h2>
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<li>Helped to start Consort, Albertas first high school iGEM team along with the Calgary Entreprenurial team, and helped them to bring home the human practices award</li>
</ul>
</ul>

Revision as of 03:37, 28 September 2013

Achievements

For our Sensor we...

  • Designed a modular platform system which allows the interchangeability of a DNA binding protein, creating the potential for multiple different DNA sensors to be developed from the framework we have submitted
  • Designed a DNA binding TALE which binds specifically to regions of the shiga toxin gene stx2 which are conserved amongst a wide variety of pathogenic E. coli
  • Submitted parts for an engineered ferritin gene, a biological nanoparticle capable of both scaffolding and stabilizing proteins, as well as carrying out catalytic reactions to act as a reporter
  • Designed, constructed, expressed, and successfully purified proteins for both the DNA sensing elements of our prototype as well as the scaffolding and reporter components of the system
  • Characterized the ability and kinetic properties of ferritin protein to act as a reporter
  • Characterized the ability for our purified ferritin produced from our construct to act as a reporter in solution as well as on a nitrocellulose membrane
  • Created multiple versions of a prototype of our final system
  • Performed modelling to inform both the quantitative imputs and construction of our system, as well as visual modelling in order to communicate how our system works in addition to informing the wetlab team of
  • 3D printed components of our system in order to better understand how our parts would look at a molecular basis

For our Human Practices we...

  • Created a dialogue with multiple industry experts and potential users in the field where our product could be applied
  • Visited all steps of the processing chain in order to better understand the culture and practices of the industry
  • Used discussions with our stakeholders to inform the design of our final system from inception towards completion so that it puts industry culture and needs first and foremost
  • Designed our system with considerations towards safety, within our laboratory and in the implementation of the final prototype
  • Thought extensively about the implications of our system in terms of the profound impact on community health and economics if false positives and negatives were to occur, and have included both positive and negative controls in our system to mitigate these implications

For our Collaboration we...

  • Worked with Paris-Bettencourt in defining biosensors for the iGEM community
  • Worked in Consort Alberta over the summer in order to develop a system for detecting xylene contamination in soils
  • Reviewed all previous iGEM projects in order to categorize which developed biosensors
  • Consolidated this information into an easy to use database (SensiGEM) in order to allow for the community to search all previous sensors to gain useful insight and find useful parts when developing future projects

For our Collaboration we...

  • Continued our collaboration with our local science centre, Telus Spark, in order to educate our community about synthetic biology and create activities which would bring the science to a level which would allow for education through something fun
  • Brought basic principles of synthetic biology to our city as a whole through our participation in running activites at our cities Beakernight event
  • Gave the world an insight into the daily life of an iGEMmer as well as sharing synthetic biology through blog posts written for Genome Alberta
  • Helped to start Consort, Albertas first high school iGEM team along with the Calgary Entreprenurial team, and helped them to bring home the human practices award

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