Achievements

For our Sensor we...

• Amended two TALEs from the registry and resubmitted these parts. We optimized the TALEs such that they can be expressed in E. coli by removing the eukaryotic kozak sequence present in the original parts, codon optimizing it for E. coli and added a his-6 tag such that this protein can be purified using affinity chromatography.
• We submitted two novel reporters: beta-lactamase and ferritin. We showed that these enzymes
• We characterized beta-lactamase as a colorimetric reporter using phenol red. We also showed that beta

For our Human Practices we...

For our Informed Design we...

• We used Informed Design: We designed our project with the cattle industries culture and needs first and foremost.
• Our project began with extensive consultation with key cattle industry stakeholders who explained the industries culture and how our technology could best fit into this it. We spoke with a rancher (Bob Church), feedlot operator (Chinook Feeders), a veterinarian (Feedlot Management Services), a meat processor (Cargill), and vaccine producer (Bioniche).
• We continued to consult with these industry stakeholders throughout the development of our FerriTALE system to ensure our technology would continue to meet the needs of the industry. Their industrial experience helped us to at many points during the summer redesign and pivot our system to better fit within the industry.

For our Collaboration we...

• We collaborated with the Paris Bettencourt iGEM Team in developing a database of biosensors made in the iGEM competitions.

For our Parts we...