Intracellular model

The intracellular model attempts to simulate the synthesis and action of KillerRed using ordinary differential equations over three stages of a typical photobleaching expeiment: induction at 37ºC, storage at 4ºC to allow for protein maturation, and photobleaching at room temperature. It includes:

• mRNA synthesis and degradation
• KillerRed synthesis, maturation, and degradation
• KillerRed states: singlet, singlet excited, triplet excited, and deactivated
• Superoxide production (by KillerRed)
• Superoxide elimination (by superoxide dismutase)

Parameters:

• mRNA synthesis: adapted from Kennell and Riezman 1977.
• mRNA degradation: Bernstein, Lin, Cohen, and Lin-Chao 2002.
• KillerRed synthesis: from Kennell and Riezman 1977 and our own fluorescence data
• Superoxide production: adapted from Tsien and Waggoner 1995; Song, Hennink, Young, and Tanke 1995; and our own fluorescence data.