Team:Carnegie Mellon/Modeling

From 2013.igem.org

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<center>
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'''Intracellular model'''
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<br><br><br><br>
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https://2013.igem.org/Team:Carnegie_Mellon/KRModel
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The intracellular model attempts to simulate the synthesis and action of KillerRed using ordinary differential equations over three stages of a typical photobleaching expeiment: induction at 37degC, storage at 4degC to allow for protein maturation, and photobleaching at room temperature. It includes:
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https://2013.igem.org/Team:Carnegie_Mellon/HostPhageDynamics
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mRNA synthesis and degradation
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</center>
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KillerRed synthesis, maturation, and degradation
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KillerRed states: singlet, singlet excited, triplet excited, and deactivated
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Superoxide production (by KillerRed)
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Superoxide elimination (by superoxide dismutase)
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Parameters:
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mRNA synthesis: adapted from Kennell and Riezman 1977.
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mRNA degradation: Bernstein, Lin, Cohen, and Lin-Chao 2002.
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KillerRed synthesis: from Kennell and Riezman 1977 and our own fluorescence data
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Superoxide production: adapted from Tsien and Waggoner 1995; Song, Hennink, Young, and Tanke 1995; and our own fluorescence data.
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Revision as of 21:46, 26 September 2013

Killer Red