Team:Carnegie Mellon/Project/Procedure

From 2013.igem.org

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<h1>Procedure</h1>
<h1>Procedure</h1>
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<p>The basic goals of the project include the following:
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<ol>
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<li>Engineer a temperate phage (specifically λ) to synthesize KillerRed in <i>E. coli</i></li>
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<li>Demonstrate KillerRed's ability to kill <i>E. coli</i></li>
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[[image:Plasmid Construct.png|thumb|600px|center|The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence. ]]
[[image:Plasmid Construct.png|thumb|600px|center|The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence. ]]

Revision as of 00:03, 27 September 2013

Killer Red



Procedure

The basic goals of the project include the following:

  1. Engineer a temperate phage (specifically λ) to synthesize KillerRed in E. coli
  2. Demonstrate KillerRed's ability to kill E. coli
  3. The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence.

    This is meant to be a guide to our experimental process, including challenges we encountered from conception to results. For a detailed description of our protocols and methods we used, visit our Protocols and Notebook pages.