Team:Carnegie Mellon/Project/Procedure

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Killer Red



Procedure

The basic goals of the project include the following:

  1. Engineer a temperate phage (specifically λ) to synthesize KillerRed in E. coli
  2. Demonstrate KillerRed's ability to kill E. coli

    3. The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence.

    This is meant to be a guide to our experimental process, including challenges we encountered from conception to results. For a detailed description of our protocols and methods we used, visit our Protocols and Notebook pages.

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