Team:Carnegie Mellon/Project/Results

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Revision as of 22:47, 26 September 2013

Killer Red

Figure 1: Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on E. coli XL10 by irradiating with a HBO100 lamp (includes 375 nm LP filter) for 5 hours.

KillerRed's phototoxic effect on E. coli XL10 is shown in Figure 1.
RFP 114% ± 20% (viable cells)/(Normalized RFU)
KillerRed: 53% ± 17% (viable cells)/(Normalized RFU)

Figure 1: Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp

XL10 Ultracompetent cells were transformed with KillerRed (BBa_K1184000) cloned with <partinfo>BBa_B0034</partinfo> as the RBS and <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.

**Table 1** Tecan Safire² Parameters
Excitation (nm)	585
Emission (nm)	610
Excitation bandwidth (nm)	10
Emission bandwidth (nm)	10
Gain	129
Number of reads	10
Integration Time (microseconds)	40

**Table 2** Shows the fluorescence data over time during photobleaching.
Time (minutes)	Fluorescence (RFU)
0	42598
20	37616
40	33749
60	29059
80	25680
100	21985
120	19442
140	17031
160	15738
180	13741

@@ Line 1: / Line 1: @@
 {{:Team:Carnegie_Mellon/Templates/header}}
 <br>
-[[file:KillerRed_RFP_Phototoxicity1.png|thumb|600px|center|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a  HBO100 lamp (includes 375 nm LP filter) for 5 hours.]]
+[[image:KillerRed RFP Phototoxicity1.png|thumb|600px|center|<b>Figure 1:</b> Phototoxicity of KillerRed and mRFP1 (<partinfo>BBa_E1010</partinfo>) on <i>E. coli</i> XL10 by irradiating with a  HBO100 lamp (includes 375 nm LP filter) for 5 hours.]]
 <p>KillerRed's phototoxic effect on <i>E. coli</i> XL10 is shown in Figure 1.<br />
 RFP 114% ± 20%  (viable cells)/(Normalized RFU) <br />
 KillerRed: 53% ± 17% (viable cells)/(Normalized RFU)
 </p>
-[[file:KR-Photobleach.png|thumb|600px|center|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]]
+[[image:KR-Photobleach.png|thumb|600px|center|<b>Figure 1:</b> Photobleaching curve of KillerRed with a HBO100 mercury-arc lamp]]
 <p>XL10 Ultracompetent cells were transformed with KillerRed (BBa_K1184000) cloned with <partinfo>BBa_B0034</partinfo> as the RBS and  <partinfo>BBa_R0010</partinfo> as the wild-type lac promoter and induced overnight with IPTG.The overnight was bleached for 180 minutes with HBO100 (100W Mercury-arc lamp). Fluorescence data was taken using a Tecan Safire II with the parameters shown in Table 1. Fluorescence Data is shown in Table 2.</p>
 <table cellpadding="2" border="1px" cellspacing="0" align="center" width="40%">