Team:Chiba/Assay/oxidation

From 2013.igem.org

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<h2>Experiment for measurement of oxidative state</h2>
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<h2>oxidation実験方法</h2>
 
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1.メチレンブルーを蒸留水に溶かし、10mMメチレンブルー溶液を作製した<br>
 
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2.メチレンブルー溶液をフィルター滅菌した<br>
 
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3.メチレンブルーを以下のように希釈してから、125%LB培地32mLに希釈したメチレンブルー8mLを混ぜた<br>
 
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4.12時間プレ培したBL21とshuffleをメチレンブループレートに植菌した<br>
 
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<br>1. Dissolved methylene blue into distilled water and made 10 mM methylene blue solution.
<br>1. Dissolved methylene blue into distilled water and made 10 mM methylene blue solution.
<br>2. Sterilized methylene blue solution using a filter.
<br>2. Sterilized methylene blue solution using a filter.
<br>3. Took 8mL of diluted methylene blue solution as shown in Fig.X and mixed with 125% LB culture 32mL.
<br>3. Took 8mL of diluted methylene blue solution as shown in Fig.X and mixed with 125% LB culture 32mL.
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<br>4.  precultured BL21and shuffle for 12 hours being cultured on methylene blue plates.
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Revision as of 01:54, 28 September 2013

iGEM-2013 Chiba

iGEM-2013 Chiba

Experiment for measurement of oxidative state



1. Dissolved methylene blue into distilled water and made 10 mM methylene blue solution.
2. Sterilized methylene blue solution using a filter.
3. Took 8mL of diluted methylene blue solution as shown in Fig.X and mixed with 125% LB culture 32mL.
4. precultured BL21and shuffle for 12 hours being cultured on methylene blue plates.