Team:Ciencias-UNAM/Project/GeneralDesign

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<div id="u46_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">Home &gt; Project &gt; General Design</span></p></div>
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<p style="text-align:left;"><span style="font-family:Arial;font-size:18px;font-weight:bold;font-style:normal;text-decoration:none;color:#666666;">
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General Design</span></p>
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<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">
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<p align="justify">
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Our design has two systems:
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<br>The <b>Antimicrobial Peptide</b> System, which produces the peptide LL-37; and
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<br>The <b>Resistance and Expulsion</b> System which produces the MarA protein, which activates the arcAB & TolC operons, activating the arcAB & TolC efflux pump.
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<p><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">
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Antimicrobial Peptide System</span></p>
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<p align="justify">
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<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">
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<i>H. pylori</i> and other pathogenic bacteria produce AI-2, a <i>quorum sensing</i> molecule. This signal is detected by the pLsrA promoter <a href=" http://parts.igem.org/wiki/index.php?title=Part:BBa_K117002">(BBa_K117002)</a>, which allows the transcription of LL37 <a href="http://parts.igem.org/Part:BBa_K1230001">(BBa_K1230001)</a>, which is traduced and then translated into the antimicrobial peptide LL37. Once LL37 is expelled by the ArcAB-TolC efflux pump, it can act against <i>H. pylori</i>.
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<br> <span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">
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<br>Resistance & Expulsion System
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<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">
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This system is induced by promoter LacI <a href="http://parts.igem.org/Part:BBa_R0010">(BBa_R0010)</a>, which allows the transcription of marA gene <a href="http://parts.igem.org/Part:BBa_K1230000">(BBa_K1230000)</a>, producing the protein MarA. MarA acts on the arcAB and tolC operons, which activates de ArcAB-TolC efflux pump, allowing the expulsion of LL37 peptide.
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<p style="text-align:left;"><span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;text-decoration:none;">&nbsp;</span></p><p style="text-align:left;">
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<span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">
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Why <i>Skully coli</i>?
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<span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">
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Mexican culture has an interesting relationship with death. There’s a holiday called <b>The Day of the Death</b>, (or <i>Día de muertos</i> in Spanish) in which the deceased are celebrated with colorfully decorated altars with sugar skulls, food, beverages and some of the favorite belongings of the deceased.
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<br>
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<br>
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The protagonist of this celebration is Death itself.  Death has different names (curiously enough, all of them feminine) which include: <i>La Parca</i>, <i>La Huesuda</i>, <i>La Calaca</i>, <i>La Calavera</i> and <i>La Catrina</i> (this last one is a name that Diego Rivera gave to a famous zinc etching by Jose Guadalupe Posada, portraying an <i>“elegant skull”</i>)
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<br>
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<br>
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An interesting characteristic of  <i>La Catrina</i> is that she serves as an intermediary in the death of a “chosen one”, meaning it only takes a specific victim. Our synthetic design intends to modify a bacteria so it can go after specific pathogenic bacteria <i>in vivo</i>. We though of the name <i>Skully coli</i>  as a fun cultural analogy that also describes the intention of our system.
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<div id="u48_rtf"><p style="text-align:left;"><span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">PROJECT MENU</span></p></div>
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<div id="u49_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Overview</span></p></div>
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<div id="u50_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">General Design</span></p></div>
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<div id="u51_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Wet Lab</span></p></div>
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<div id="u52_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">BioBricks</span></p></div>
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<div id="u53_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Results</span></p></div>
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<div id="u54_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Characterization</span></p></div>
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<div id="u55_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Notebook</span></p></div>
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<div id="u56_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Dry Lab</span></p></div>
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<div id="u57_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Modelling</span></p></div>
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<div id="u58_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">Conclusions </span></p></div>
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<div id="u59_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:normal;font-style:normal;text-decoration:none;color:#929EA0;">Future Work</span></p></div>
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<div id="u60_rtf"><p style="text-align:left;"><span style="font-family:Arial;font-size:14px;font-weight:bold;font-style:normal;text-decoration:none;color:#929EA0;">References</span></p></div>
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Latest revision as of 04:14, 27 September 2013

General Design

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General Design

Our design has two systems:
The Antimicrobial Peptide System, which produces the peptide LL-37; and
The Resistance and Expulsion System which produces the MarA protein, which activates the arcAB & TolC operons, activating the arcAB & TolC efflux pump.

Antimicrobial Peptide System

H. pylori and other pathogenic bacteria produce AI-2, a quorum sensing molecule. This signal is detected by the pLsrA promoter (BBa_K117002), which allows the transcription of LL37 (BBa_K1230001), which is traduced and then translated into the antimicrobial peptide LL37. Once LL37 is expelled by the ArcAB-TolC efflux pump, it can act against H. pylori.

Resistance & Expulsion System

This system is induced by promoter LacI (BBa_R0010), which allows the transcription of marA gene (BBa_K1230000), producing the protein MarA. MarA acts on the arcAB and tolC operons, which activates de ArcAB-TolC efflux pump, allowing the expulsion of LL37 peptide.

 

Why Skully coli?

Mexican culture has an interesting relationship with death. There’s a holiday called The Day of the Death, (or Día de muertos in Spanish) in which the deceased are celebrated with colorfully decorated altars with sugar skulls, food, beverages and some of the favorite belongings of the deceased.

The protagonist of this celebration is Death itself. Death has different names (curiously enough, all of them feminine) which include: La Parca, La Huesuda, La Calaca, La Calavera and La Catrina (this last one is a name that Diego Rivera gave to a famous zinc etching by Jose Guadalupe Posada, portraying an “elegant skull”)

An interesting characteristic of La Catrina is that she serves as an intermediary in the death of a “chosen one”, meaning it only takes a specific victim. Our synthetic design intends to modify a bacteria so it can go after specific pathogenic bacteria in vivo. We though of the name Skully coli as a fun cultural analogy that also describes the intention of our system.

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