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Team:DTU-Denmark/Bioreactor
From 2013.igem.org
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| - | Nitrous Oxide | + | Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment. |
| - | {| | + | {| class="wikitable" |
!Time | !Time | ||
!Replicate 1 | !Replicate 1 | ||
!Replicate 2 | !Replicate 2 | ||
!Replicate 3 | !Replicate 3 | ||
| + | |Control: Distilled Water | ||
|- | |- | ||
|14:00 | |14:00 | ||
| - | | | + | | 1.84 mg/L |
| - | | | + | | 1.64 mg/L |
| - | | | + | | 1.60 mg/L |
|- | |- | ||
|22:00 | |22:00 | ||
| - | | | + | | 1.43 mg/L |
| - | | | + | | 1.44 mg/L |
| - | | | + | | 1.50 mg/L |
| + | | 1.65 mg/L | ||
|} | |} | ||
| + | == Discussion == | ||
| + | |||
| + | There were several sources of error in this experiment. Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors. | ||
<div style="clear: both;"></div> | <div style="clear: both;"></div> | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Revision as of 15:04, 28 September 2013
Bioreactor
Contents |
Description
The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.
Methods
Protocol Bioreactor
Results
Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.
| Time | Replicate 1 | Replicate 2 | Replicate 3 | Control: Distilled Water |
|---|---|---|---|---|
| 14:00 | 1.84 mg/L | 1.64 mg/L | 1.60 mg/L | |
| 22:00 | 1.43 mg/L | 1.44 mg/L | 1.50 mg/L | 1.65 mg/L |
Discussion
There were several sources of error in this experiment. Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.
