Team:DTU-Denmark/Experiment2

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{{:Team:DTU-Denmark/Templates/StartPage|Experiment 2}}
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{{:Team:DTU-Denmark/Templates/StartPage|Production of nitrous oxide (N<sub>2</sub>O) from nitrite (NO<sub>2</sub><sup>-</sup> ) under anaerobic conditions}}
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== Description ==
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== Introduction ==
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'''Production of nitrous oxide (N<sub>2</sub>O) from nitrite (NO<sub>2</sub><sup>-</sup> ) under anaerobic conditions'''
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In this anaerobic experiment we add nitrite  (NO<sub>2</sub><sup>-</sup> ) in a series of spikes to an untransformed strain of ''E. coli'' and to an untransformed strain of ''P. aeruginosa'' in order to characterize how readily nitrite is converted to nitric oxide (NO), and subsequently to nitrous oxide (N<sub>2</sub>O). We also monitor nitrite, ammonia and nitrate concentrations before each spike.  The concentrations of both gasses are measured continually, and the ammonia, nitrite and nitrate concentrations are measured at a series of time points around the spike.
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The behavior of untransformed ''E. coli'' and untransformed ''P. aeruginosa'' is characterized while growing in the presence of NO<sub>2</sub><sup>-</sup> in an anaerobic reaction chamber by measuring the conversion of nitrite (NO<sub>2</sub><sup>-</sup>) to nitric oxide (NO) and nitrous oxide (N<sub>2</sub>O).
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In this anaerobic experiment we add nitrite in a series of spikes to an untransformed strain of ''E. coli'' and to an untransformed strain of ''P. aeruginosa'' in order to test how readily nitrite is converted to nitric oxide, and subsequently to nitrous oxide. We also monitor nitrite, ammonia and nitrate concentrations before each spike.  The concentrations of both gasses are measured continually, and the ammonia, nitrite and nitrate concentrations will be measured after nitrite is spiked.
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==Methods==
==Methods==

Revision as of 16:22, 28 September 2013

Production of nitrous oxide (N2O) from nitrite (NO2- ) under anaerobic conditions

Contents

Introduction

In this anaerobic experiment we add nitrite (NO2- ) in a series of spikes to an untransformed strain of E. coli and to an untransformed strain of P. aeruginosa in order to characterize how readily nitrite is converted to nitric oxide (NO), and subsequently to nitrous oxide (N2O). We also monitor nitrite, ammonia and nitrate concentrations before each spike. The concentrations of both gasses are measured continually, and the ammonia, nitrite and nitrate concentrations are measured at a series of time points around the spike.

Methods

For the calibration of the electrodes we follow the Calibration protocol

The procedure is based on Experiment 2

Results

Round 1

DTU-Experiment-2-results.png

Mg of Nitrogen (as nitrite or nitrous oxide) per litre measured for varying concentrations of Nitrite added at t=0. All experiments were performed anaerobically.


Round 2

Dtu Experiment 2 round 2 --sample A.png Dtu Experiment 2 round 2 -- sample B.png