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Team:DTU-Denmark/Experiment2
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In this anaerobic experiment we add nitrite in a series of spikes to a native strain of ''E. coli'' and to a native strain of ''P. aeruginosa'' in order to test how readily nitrite is converted to nitric oxide, and subsequently to nitrous oxide. We also monitor nitrite, ammonia and nitrate concentrations before each spike. The concentrations of both gasses are measured continually, and the ammonia, nitrite and nitrate concentrations will be measured after nitrite is spiked. | In this anaerobic experiment we add nitrite in a series of spikes to a native strain of ''E. coli'' and to a native strain of ''P. aeruginosa'' in order to test how readily nitrite is converted to nitric oxide, and subsequently to nitrous oxide. We also monitor nitrite, ammonia and nitrate concentrations before each spike. The concentrations of both gasses are measured continually, and the ammonia, nitrite and nitrate concentrations will be measured after nitrite is spiked. | ||
| + | ==Methods== | ||
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| + | Using Protocol [[Team:DTU-Denmark/Methods/Experiment_2|Experiment 2]] | ||
== Results == | == Results == | ||
Revision as of 17:09, 12 August 2013
Experiment 2
Contents |
Description
Measuring the production of N2O from Nitrite (NO2- ) anaerobically
The behavior of E. coli (untransformed cells) and P. aeruginosa is characterized while growing in the presence of NO2- in an anaerobic reaction chamber by measuring the conversion of nitrite (NO2-) to nitric oxide (NO) and nitrous oxide (N2O).
In this anaerobic experiment we add nitrite in a series of spikes to a native strain of E. coli and to a native strain of P. aeruginosa in order to test how readily nitrite is converted to nitric oxide, and subsequently to nitrous oxide. We also monitor nitrite, ammonia and nitrate concentrations before each spike. The concentrations of both gasses are measured continually, and the ammonia, nitrite and nitrate concentrations will be measured after nitrite is spiked.
Methods
Using Protocol Experiment 2
Results
Mg of Nitrogen (as nitrite or nitrous oxide) per litre measured for varying concentrations of Nitrite added at t=0. All experiments were performed anaerobically.
