Team:DTU-Denmark/Experiment2
From 2013.igem.org
Line 3: | Line 3: | ||
== Description == | == Description == | ||
- | ''' | + | '''Production of nitrous oxide (N<sub>2</sub>O) from nitrite (NO<sub>2</sub><sup>-</sup> ) under anaerobic conditions''' |
- | The behavior of ''E. coli'' | + | The behavior of untransformed ''E. coli'' and untransformed ''P. aeruginosa'' is characterized while growing in the presence of NO<sub>2</sub><sup>-</sup> in an anaerobic reaction chamber by measuring the conversion of nitrite (NO<sub>2</sub><sup>-</sup>) to nitric oxide (NO) and nitrous oxide (N<sub>2</sub>O). |
- | In this anaerobic experiment we add nitrite in a series of spikes to | + | In this anaerobic experiment we add nitrite in a series of spikes to an untransformed strain of ''E. coli'' and to an untransformed strain of ''P. aeruginosa'' in order to test how readily nitrite is converted to nitric oxide, and subsequently to nitrous oxide. We also monitor nitrite, ammonia and nitrate concentrations before each spike. The concentrations of both gasses are measured continually, and the ammonia, nitrite and nitrate concentrations will be measured after nitrite is spiked. |
==Methods== | ==Methods== |
Revision as of 16:19, 28 September 2013
Experiment 2
Contents |
Description
Production of nitrous oxide (N2O) from nitrite (NO2- ) under anaerobic conditions
The behavior of untransformed E. coli and untransformed P. aeruginosa is characterized while growing in the presence of NO2- in an anaerobic reaction chamber by measuring the conversion of nitrite (NO2-) to nitric oxide (NO) and nitrous oxide (N2O).
In this anaerobic experiment we add nitrite in a series of spikes to an untransformed strain of E. coli and to an untransformed strain of P. aeruginosa in order to test how readily nitrite is converted to nitric oxide, and subsequently to nitrous oxide. We also monitor nitrite, ammonia and nitrate concentrations before each spike. The concentrations of both gasses are measured continually, and the ammonia, nitrite and nitrate concentrations will be measured after nitrite is spiked.
Methods
For the calibration of the electrodes we follow the Calibration protocol
The procedure is based on Experiment 2
Results
Round 1
Mg of Nitrogen (as nitrite or nitrous oxide) per litre measured for varying concentrations of Nitrite added at t=0. All experiments were performed anaerobically.
Round 2