Introduction

Since we are working with many periplasmic proteins, we wanted to do an initial project to try to target proteins to the periplasm. To do this, we used periplasmic signal peptides from the TAT and Sec pathways, and with a translational fusion of the signal peptide to GFP, we expressed GFP in the periplasm. Simultaneously, we expressed RFP in the cytoplasm.

Results

We have created the biobrick XXX

Link to the lab book pages