Team:DTU-Denmark/Kinetic Model

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Kinetic model of the Pathway

We are applying kinetic modeling to investigate how long it takes a cell to produce a certain amount of nitrous oxide. The reactions of the pathway we are trying to integrate in E. coli are:

Mutant 1: NH3 → NH2OH → NO2

Mutant 2: NO2 → NO → N2O

The iGEM team from Taipei in 2012 was using Nir and Nos in their project as well and did some kinetic modeling based on literature research. To describe product formation by the enzymes they used the Michaelis-Menten approach:

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Some of the necessary parameters can be found in literature, they are listed in Table 1.

It is necessary to know or estimate the enzyme concentration if kcat values are used. The Taipeh Team did this with another kinetic model, but we are missing information about many of this models parameters so the result would be very inexact. Therefore we chose to use four different enzyme concentrations in our model: 100, 500, 1000 and 100 000 enzymes per cell corresponding to low, medium, high and very high concentrations of enzyme. These numbers are based on [4].

The reactor

References