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Team:DTU-Denmark/Methods/Ammonium colorimetric measurements
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{{:Team:DTU-Denmark/Templates/StartPage|Ammonium colorimetric measurements}} | {{:Team:DTU-Denmark/Templates/StartPage|Ammonium colorimetric measurements}} | ||
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==Ammonium colorimetric measurements== | ==Ammonium colorimetric measurements== | ||
| - | + | '''Measuring the concentration of NH<sub>4</sub> in an ''E. coli''/''P. aeruginosa'' growth experiment using Spectroquant kit | |
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| - | '''Measuring the concentration of NH<sub>4</sub> in an ''E. coli''/''P. | + | |
''' | ''' | ||
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* Ammonium Test Kit (#00683) | * Ammonium Test Kit (#00683) | ||
| - | A liquid test solution should be performed to achieve a measurement between the ranges of 2-75 mg/L | + | A liquid test solution should be performed to achieve a measurement between the ranges of 2-75 mg/L NH<sub>4</sub>-N. |
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Ammonium solution | Ammonium solution | ||
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Prepare solution with 39 mg NH<sub>4</sub>-N/L: | Prepare solution with 39 mg NH<sub>4</sub>-N/L: | ||
* Weigh 220 mg Ammonium Bicarbonate into 100mL volumetric flask (10x concentrated) | * Weigh 220 mg Ammonium Bicarbonate into 100mL volumetric flask (10x concentrated) | ||
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Performing Measurements: | Performing Measurements: | ||
'''Ammonium''' | '''Ammonium''' | ||
| - | Measuring range of the kit: 2-75 mg/L NH<sub>4</sub>-N | + | |
| + | ''Measuring range of the kit: 2-75 mg/L NH<sub>4</sub>-N'' | ||
# Pipette 5.0 ml of reagent 1 (NH<sub>4</sub>-1) into a test tube. | # Pipette 5.0 ml of reagent 1 (NH<sub>4</sub>-1) into a test tube. | ||
# Add 0.20 mL of sample when it is among the range of measuring kit (otherwise make proper dilution) | # Add 0.20 mL of sample when it is among the range of measuring kit (otherwise make proper dilution) | ||
| - | # Add 1 level spoon of reagent 2 ( | + | # Add 1 level spoon of reagent 2 (NH<sub>4</sub>-2). |
# Shake vigorously to dissolve. | # Shake vigorously to dissolve. | ||
# Reaction time: 15 mins | # Reaction time: 15 mins | ||
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# Note the result | # Note the result | ||
# If the read is above the linear range then put the sample back to the tube test, dilute it and try again. | # If the read is above the linear range then put the sample back to the tube test, dilute it and try again. | ||
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Latest revision as of 18:09, 4 October 2013
Ammonium colorimetric measurements
Ammonium colorimetric measurements
Measuring the concentration of NH4 in an E. coli/P. aeruginosa growth experiment using Spectroquant kit
Material:
- Ammonium Test Kit (#00683)
A liquid test solution should be performed to achieve a measurement between the ranges of 2-75 mg/L NH4-N.
Ammonium solution
Prepare solution with 39 mg NH4-N/L:
- Weigh 220 mg Ammonium Bicarbonate into 100mL volumetric flask (10x concentrated)
- Take 5 mL of the 10x solution in 45 mL water
- Expected final concentration 39 mg N/L
Performing Measurements: Ammonium
Measuring range of the kit: 2-75 mg/L NH4-N
- Pipette 5.0 ml of reagent 1 (NH4-1) into a test tube.
- Add 0.20 mL of sample when it is among the range of measuring kit (otherwise make proper dilution)
- Add 1 level spoon of reagent 2 (NH4-2).
- Shake vigorously to dissolve.
- Reaction time: 15 mins
- Pour sample into the cuvette
- Select method in the spectrometer by entering the AutoSelector (little black tube)
- Note the result
- If the read is above the linear range then put the sample back to the tube test, dilute it and try again.
