Team:DTU-Denmark/Methods/Modified DM minimal medium

From 2013.igem.org

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'''1 L of Modified DM minimal medium '''
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{| class="wikitable sortable" style="text-align: right"
{| class="wikitable sortable" style="text-align: right"
! substances !! Amount (g)
! substances !! Amount (g)
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| Trace elements || 10 ml
| Trace elements || 10 ml
|}
|}
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'''10 ml trace element solution'''
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{| class="wikitable sortable" style="text-align: right"
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! substances !! Concentration (mg/L)
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|-
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| FeCl<sub>3</sub>•6H<sub>2</sub>O || 13.5
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|-
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| Na<sub>2</sub>MoO<sub>4</sub>•2H<sub>2</sub>O|| 0.6
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|-
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| MgSO<sub>4</sub> || 3.4
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|-
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| CoCl<sub>2</sub>•6H<sub>2</sub>O || 0.6
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|-
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| MnCl<sub>2</sub>•4H<sub>2</sub>O || 1
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|-
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| In that liter we add 10 ml
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|-
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| Trace elements || 10 ml
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|}
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Revision as of 10:05, 13 August 2013

Autoclaving

1 L of Modified DM minimal medium

substances Amount (g)
potassium hydrogenphophate (K2HPO4 7
glucose (C6H12O6) 4.89
magnesium sulfate (MgSO4) 0.2
sodium citrate (Na3C6H5O7•2H2O) 0.5
potassium dihydrogenphosphate (KH2PO4) 3
In that liter we add 10 ml
Trace elements 10 ml

10 ml trace element solution

substances Concentration (mg/L)
FeCl3•6H2O 13.5
Na2MoO4•2H2O 0.6
MgSO4 3.4
CoCl2•6H2O 0.6
MnCl2•4H2O 1
In that liter we add 10 ml
Trace elements 10 ml

Retrieved from "http://2013.igem.org/Team:DTU-Denmark/Methods/Modified_DM_minimal_medium"