Team:DTU-Denmark/Methods/Modified DM minimal medium

From 2013.igem.org

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'''1 L of Modified DM minimal medium '''
'''1 L of Modified DM minimal medium '''
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! substances !! Amount (g)
! substances !! Amount (g)
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| potassium hydrogenphophate (K<sub>2</sub>HPO<sub>4</sub> || 7  
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| potassium hydrogenphophate (K<sub>2</sub>HPO<sub>4</sub>) || 7  
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| glucose (C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>)|| 4.89  
| glucose (C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>)|| 4.89  
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| potassium dihydrogenphosphate (KH<sub>2</sub>PO<sub>4</sub>) || 3
| potassium dihydrogenphosphate (KH<sub>2</sub>PO<sub>4</sub>) || 3
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| In that liter we add 10 ml  
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| '''In that liter we add 10 ml'''
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| Trace elements || 10 ml
| Trace elements || 10 ml
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| MnCl<sub>2</sub>•4H<sub>2</sub>O || 1
| MnCl<sub>2</sub>•4H<sub>2</sub>O || 1
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| In that liter we add 10 ml
 
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| Trace elements || 10 ml
 
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Revision as of 10:07, 13 August 2013

Modified DM minimal medium

1 L of Modified DM minimal medium

substances Amount (g)
potassium hydrogenphophate (K2HPO4) 7
glucose (C6H12O6) 4.89
magnesium sulfate (MgSO4) 0.2
sodium citrate (Na3C6H5O7•2H2O) 0.5
potassium dihydrogenphosphate (KH2PO4) 3
In that liter we add 10 ml
Trace elements 10 ml

10 ml trace element solution

substances Concentration (mg/L)
FeCl3•6H2O 13.5
Na2MoO4•2H2O 0.6
MgSO4 3.4
CoCl2•6H2O 0.6
MnCl2•4H2O 1

Retrieved from "http://2013.igem.org/Team:DTU-Denmark/Methods/Modified_DM_minimal_medium"