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Team:DTU-Denmark/Methods/Modified DM minimal medium
From 2013.igem.org
(Difference between revisions)
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{| class="wikitable sortable" style="text-align: right" | {| class="wikitable sortable" style="text-align: right" | ||
| - | ! | + | ! Substances !! Amount (g) |
|- | |- | ||
| - | | potassium | + | | potassium hydrogenphosphate (K<sub>2</sub>HPO<sub>4</sub>) || 7 |
|- | |- | ||
| glucose (C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>)|| 4.89 | | glucose (C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>)|| 4.89 | ||
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{| class="wikitable sortable" style="text-align: right" | {| class="wikitable sortable" style="text-align: right" | ||
| - | ! | + | ! Substances !! Concentration (mg/L) |
|- | |- | ||
| FeCl<sub>3</sub>•6H<sub>2</sub>O || 13.5 | | FeCl<sub>3</sub>•6H<sub>2</sub>O || 13.5 | ||
Revision as of 10:08, 13 August 2013
Modified DM minimal medium
1 L of Modified DM minimal medium
| Substances | Amount (g) |
|---|---|
| potassium hydrogenphosphate (K2HPO4) | 7 |
| glucose (C6H12O6) | 4.89 |
| magnesium sulfate (MgSO4) | 0.2 |
| sodium citrate (Na3C6H5O7•2H2O) | 0.5 |
| potassium dihydrogenphosphate (KH2PO4) | 3 |
| In that liter we add 10 ml | |
| Trace elements | 10 ml |
10 ml trace element solution
| Substances | Concentration (mg/L) |
|---|---|
| FeCl3•6H2O | 13.5 |
| Na2MoO4•2H2O | 0.6 |
| MgSO4 | 3.4 |
| CoCl2•6H2O | 0.6 |
| MnCl2•4H2O | 1 |
