Team:DTU-Denmark/Methods/Modified DM minimal medium
From 2013.igem.org
(Difference between revisions)
(3 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | {{:Team:DTU-Denmark/Templates/StartPage| | + | {{:Team:DTU-Denmark/Templates/StartPage|Modified DM minimal medium}} |
+ | <div class="overviewPage"> | ||
'''1 L of Modified DM minimal medium ''' | '''1 L of Modified DM minimal medium ''' | ||
{| class="wikitable sortable" style="text-align: right" | {| class="wikitable sortable" style="text-align: right" | ||
- | ! | + | ! Substances !! Amount (g) |
|- | |- | ||
- | | potassium | + | | potassium hydrogenphosphate (K<sub>2</sub>HPO<sub>4</sub>) || 7 |
|- | |- | ||
| glucose (C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>)|| 4.89 | | glucose (C<sub>6</sub>H<sub>12</sub>O<sub>6</sub>)|| 4.89 | ||
Line 16: | Line 17: | ||
| potassium dihydrogenphosphate (KH<sub>2</sub>PO<sub>4</sub>) || 3 | | potassium dihydrogenphosphate (KH<sub>2</sub>PO<sub>4</sub>) || 3 | ||
|- | |- | ||
- | | In that liter we add 10 ml | + | | '''In that liter we add 10 ml''' |
|- | |- | ||
| Trace elements || 10 ml | | Trace elements || 10 ml | ||
Line 24: | Line 25: | ||
{| class="wikitable sortable" style="text-align: right" | {| class="wikitable sortable" style="text-align: right" | ||
- | ! | + | ! Substances !! Concentration (mg/L) |
|- | |- | ||
| FeCl<sub>3</sub>•6H<sub>2</sub>O || 13.5 | | FeCl<sub>3</sub>•6H<sub>2</sub>O || 13.5 | ||
Line 35: | Line 36: | ||
|- | |- | ||
| MnCl<sub>2</sub>•4H<sub>2</sub>O || 1 | | MnCl<sub>2</sub>•4H<sub>2</sub>O || 1 | ||
- | |||
- | |||
- | |||
- | |||
|} | |} | ||
- | {{:Team:DTU-Denmark/Templates/ | + | <div style="clear: both;"></div> |
+ | </div> | ||
+ | {{:Team:DTU-Denmark/Templates/EndPage}} |
Latest revision as of 17:50, 15 August 2013
Modified DM minimal medium
1 L of Modified DM minimal medium
Substances | Amount (g) |
---|---|
potassium hydrogenphosphate (K2HPO4) | 7 |
glucose (C6H12O6) | 4.89 |
magnesium sulfate (MgSO4) | 0.2 |
sodium citrate (Na3C6H5O7•2H2O) | 0.5 |
potassium dihydrogenphosphate (KH2PO4) | 3 |
In that liter we add 10 ml | |
Trace elements | 10 ml |
10 ml trace element solution
Substances | Concentration (mg/L) |
---|---|
FeCl3•6H2O | 13.5 |
Na2MoO4•2H2O | 0.6 |
MgSO4 | 3.4 |
CoCl2•6H2O | 0.6 |
MnCl2•4H2O | 1 |