Team:DTU-Denmark/Methods/PCR

From 2013.igem.org

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1. 98°C for 2:00 \linebreak
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== Generel example on a normal PCR-program ==
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2. 98°C for 0:10
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# 98°C for 2:00
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# 98°C for 0:10
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# 59°C for 0:30
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# 72°C for 2:00
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# Go to number 2 - repeat 35
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# 72°C for 5:00
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# End
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Always use heated lid and set the machine to wait until the lid have the right temperature. If not default set to lid temperature to 104°C.

Revision as of 17:54, 26 June 2013

Generel example on a normal PCR-program

  1. 98°C for 2:00
  2. 98°C for 0:10
  3. 59°C for 0:30
  4. 72°C for 2:00
  5. Go to number 2 - repeat 35
  6. 72°C for 5:00
  7. End

Always use heated lid and set the machine to wait until the lid have the right temperature. If not default set to lid temperature to 104°C.

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