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Team:DTU-Denmark/Methods/USER Cloning and Transformation
From 2013.igem.org
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#Label 1,5 mL Eppendorf tubes and place in freezer, remove LB-Amp plates from fridge and place at room temperature. | #Label 1,5 mL Eppendorf tubes and place in freezer, remove LB-Amp plates from fridge and place at room temperature. | ||
| - | #Mix the USER cloning | + | #Mix the USER cloning <sup>TM</sup> |
Revision as of 12:22, 6 September 2013
Materials
PCR product(s) 10X diluted BSA NEB buffer 4 USER enzyme 1 PCR tube/reaction 1 Eppendorf tube/reaction 100 uL competent E.coli/reaction 1 LB-AMP plate/reaction Drigalsky's spatchula or sterile glass beads Milli-Q water 96% Ethanol Gas Burner
Procedure
- Label 1,5 mL Eppendorf tubes and place in freezer, remove LB-Amp plates from fridge and place at room temperature.
- Mix the USER cloning TM
