"
Team:DTU-Denmark/Notebook
From 2013.igem.org
(Difference between revisions)
| Line 34: | Line 34: | ||
===July=== | ===July=== | ||
| + | Week 27 | ||
| + | * Hello World project is successful. From a cytoplasmic extraction, we have observed GFP in the periplasm and RFP in the cytoplasm. See a nice picture [[Team:DTU-Denmark/Notebook/6_July_2013|here.]] | ||
* 2013-07-06 "Hello World" pilot project complete | * 2013-07-06 "Hello World" pilot project complete | ||
| + | * Biolector experiment complete, initial data analysis complete. See some graphs [[Team:DTU-Denmark/ToxicityExperiment|here]] | ||
| - | + | Week 28 | |
| - | ** | + | * Made media and began growing ''Nitrosomonas'' culture |
| + | * Designed, ordered and received primers to extract genes from ''Nitrosomonas'' and ''Pseudomonas'' | ||
| + | * Began work on cloning genes out of ''Nitrosomonas europaea'' and ''Pseudomonas aeruginosa''. All isolations successful except Nir. | ||
| - | * | + | Week 29 |
| - | ** | + | * Performed first nitrate -> nitrite experiment |
| - | ** | + | |
| + | Week 30 | ||
| + | * Only have colonies for the cytochromes -- other genes do not give cultures | ||
| + | * Will try to extract Nir gene in two pieces | ||
| + | * Began work on arabinose inducible system | ||
| + | |||
| + | Week 31 | ||
| + | * Began work on Synthetic Promoter Library | ||
| + | * BBQ with KU this Saturday | ||
===August=== | ===August=== | ||
| + | |||
| + | Week 32 | ||
| + | * | ||
| + | |||
| + | Week 33 | ||
| + | |||
| + | Week 34 | ||
| + | |||
| + | Week 35 | ||
===September=== | ===September=== | ||
| + | |||
| + | Week 36 | ||
| + | |||
| + | Week 37 | ||
| + | |||
| + | Week 38 | ||
| + | |||
| + | Week 39 | ||
| + | |||
| + | === October === | ||
| + | |||
| + | Week 40 | ||
| + | * Wiki complete! | ||
==Lab Book Entries== | ==Lab Book Entries== | ||
Revision as of 12:54, 28 September 2013
Notebook
Contents |
Timeline
January
- 2013-01-15 Budget completed
- 2013-01-29 Human Practices: Workshop for highschool students
February
- 2013-02-25 Project selected: Conversion of ammonia to nitrous oxide in E. coli
March
- 2013-03-11 Biobrick workshop hosted for KU and SDU
- 2013-03-13 Project kickoff meeting
April
- 2013-04-15 Team contract signed
- 2013-04-28 Human Practices: Outreach for highschool students
May
- 2013-05-29 "Hello World" pilot project kicked off
June
- 2013-06-04 Team competency mapping
- 2013-06-09 Work on wiki started
- 2013-06-29 Team BBQ
July
Week 27
- Hello World project is successful. From a cytoplasmic extraction, we have observed GFP in the periplasm and RFP in the cytoplasm. See a nice picture here.
- 2013-07-06 "Hello World" pilot project complete
- Biolector experiment complete, initial data analysis complete. See some graphs here
Week 28
- Made media and began growing Nitrosomonas culture
- Designed, ordered and received primers to extract genes from Nitrosomonas and Pseudomonas
- Began work on cloning genes out of Nitrosomonas europaea and Pseudomonas aeruginosa. All isolations successful except Nir.
Week 29
- Performed first nitrate -> nitrite experiment
Week 30
- Only have colonies for the cytochromes -- other genes do not give cultures
- Will try to extract Nir gene in two pieces
- Began work on arabinose inducible system
Week 31
- Began work on Synthetic Promoter Library
- BBQ with KU this Saturday
August
Week 32
Week 33
Week 34
Week 35
September
Week 36
Week 37
Week 38
Week 39
October
Week 40
- Wiki complete!
Lab Book Entries
|
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
