"
Team:DTU-Denmark/Notebook/10 September 2013
From 2013.igem.org
(Difference between revisions)
(Created page with "{{:Team:DTU-Denmark/Templates/StartPage|10 September}} =lab 208= <hr/> ==Main purpose== <hr/> ==Who was in the lab== <hr/> ==Procedure== <hr/> ===Colony PCR to verify Nir in ...") |
(→Colony PCR to verify Nir in pZA21::ara-tight) |
||
| Line 13: | Line 13: | ||
===Colony PCR to verify Nir in pZA21::ara-tight=== | ===Colony PCR to verify Nir in pZA21::ara-tight=== | ||
| + | |||
| + | Redid colony PCR with sequencing primers instead. Two primer pairs to test for the two USER parts. Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq. | ||
| + | |||
| + | Used Q5 premix. | ||
==Results== | ==Results== | ||
Revision as of 12:05, 11 September 2013
10 September
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
Colony PCR to verify Nir in pZA21::ara-tight
Redid colony PCR with sequencing primers instead. Two primer pairs to test for the two USER parts. Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq.
Used Q5 premix.
