Team:DTU-Denmark/Notebook/10 September 2013

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10 September

Contents

lab 208


Main purpose


Who was in the lab


Procedure


Colony PCR to verify Nir in pZA21::ara-tight

Redid colony PCR with sequencing primers instead. Two primer pairs to test for the two USER parts. Pair 1: Nir_FW_4_Seq + Nir_RV_5_Seq . Pair 2: Nir_FW_13_Seq + Nir_RV_14_Seq.

Used Q5 premix.

Results


Gel

  • 1 kb ladder
  • colony 1, primer pair 1
  • colony 1, primer pair 2
  • colony 2, primer pair 1
  • colony 2, primer pair 2
  • colony 3, primer pair 1
  • colony 3, primer pair 2
  • colony 4, primer pair 1
  • colony 5, primer pair 2
  • colony 5, primer pair 1
  • colony 5, primer pair 2
  • colony 6, primer pair 1
  • colony 6, primer pair 2
  • 1 kb ladder

Colony pcr 10. sep..jpg

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