"

From 2013.igem.org

Revision as of 15:40, 25 June 2013 (view source) Berg (Talk | contribs) (→Procedure) ← Older edit		Revision as of 15:42, 25 June 2013 (view source) Berg (Talk | contribs) (→Procedure) Newer edit →
Line 26:		Line 26:
	|-		|-
	|master mix total || ||615 ul		|master mix total || ||615 ul
		+	|}
		+
		+	Every PCR tube contains
		+	{|
		+	|-
		+	|mastermix||41ul
		+	|-
		+	|FW primer||3ul
		+	|-
		+	|RV primer||3ul
		+	|-
		+	|DNA template||1ul
	|}		|}
	== Conclusion from today ==		== Conclusion from today ==

---

Revision as of 15:42, 25 June 2013

Contents 1 main purposes today 2 who were in the lab 3 Procedure 4 Conclusion from today

main purposes today

helloworld-project

• diluting primers with 1.5 ml milli Q water each
• diluting G-block with 20ul milli Q water

who were in the lab

Procedure

PCR

• 7 rxns in duplicates
• mastermix for 15 rxns

	per rxn	master
5x HF buffer	10ul	150ul
MgCl_2	0.5ul	7.5ul
dNTPs	5ul	75ul
Phusion polymerase	0.5ul	7.5ul
Milli Q water	25ul	375ul
master mix total		615 ul

Every PCR tube contains

mastermix	41ul
FW primer	3ul
RV primer	3ul
DNA template	1ul

Conclusion from today